Gene Expression Data
Assay Details
Assay
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Reference:
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J:111643
Boras-Granic K, et al., Genetic interaction between Lef1 and Alx4 is required for early embryonic development. Int J Dev Biol. 2006;50(7):601-10
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6755165
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Gene symbol:
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Alx4
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Gene name:
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aristaless-like homeobox 4
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Antibody:
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anti-Alx4
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2A/C:
embryonic day 9.0
(more )
Note: Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).
Specimen
2A/C:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: future telencephalon |
Strong |
Regionally restricted |
2A
,
2C
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Expressed in the mesenchyme surrounding the telencephalic vesicle.
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Specimen
2D/F:
embryonic day 9.0
(more )
Note: Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).
Specimen
2D/F:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: migrating neural crest |
Present |
Regionally restricted |
2D
,
2F
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Expressed in potential neural crest cell migratory tract which followed a path dorsal to the otic vesicle.
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Specimen
2J/L:
embryonic day 9.0
(more )
Note: Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).
Specimen
2J/L:
(close )
Genetic Background:
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C57BL/6
|
Age:
|
embryonic day 9.0
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Alx4; red - Lef1 (assay MGI:6755151).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: trunk mesenchyme |
Present |
Regionally restricted |
2L
,
2J
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Expressed in the mesenchymal cells surrounding the umbilical vein.
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TS14: somite |
Absent |
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2L
,
2J
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Specimen
9:
embryonic day 9.5
(more )
Note: Double labeled: green - Alx4; red - Pecam1 (assay MGI:6755166).
Specimen
9:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Alx4; red - Pecam1 (assay MGI:6755166).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: umbilical cord mesenchyme |
Present |
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9_lower right
,
9_lower left
,
9_upper left
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Expressed in mesenchymal cells surrounding the umbilical vein. No expression in in the nuclei of endothelial cells.
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