Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:297659
Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2;
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6750887
|
|
Gene symbol:
|
Pou4f2
|
|
Gene name:
|
POU domain, class 4, transcription factor 2
|
|
Antibody:
|
anti-Pou4f2
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 546
|
|
|
Results
|
Specimen
3F/G/H:
embryonic day 14.5; Pou4f2tm2Xmu/Pou4f2 +
(more  )
Note: Double labeled: green - Pou4f2 protein; red - Pou4f2 reporter (assay MGI:6750871).
Specimen
3F/G/H:
(close  )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl
|
|
Mutant Allele(s):
|
Pou4f2tm2Xmu/Pou4f2+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pou4f2 protein; red - Pou4f2 reporter (assay MGI:6750871).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
3G
,
3H
|
Expression is primarily seen in the ganglion cell layer. Some 20% of the protein expressing cells are found in the neuroblast layer; these are believed to be newly-born RGCs.
|
|
Analysis Tools
