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Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6741050
Gene symbol: Dclk1
Gene name: doublecortin-like kinase 1
Antibody: Anti-DCAMKL1
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3A: postnatal (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS28: intestinal villus Present 3A-4 , 3A-2 Coexpressed with Insm1 in a subset of enteroendocrine cells.
TS28: intestine epithelium Present 3A-4 , 3A-2 Coexpressed with Insm1 in a subset of enteroendocrine cells.
Specimen 3B: postnatal (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS28: intestinal villus Present 3B-2 , 3B-4 Expressed in brush cells, which were Insm-.
TS28: intestine epithelium Present 3B-2 , 3B-4 Expressed in brush cells, which were Insm-.
Specimen 14 Villi-1: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: intestinal villus Present 14_1-3 , 14_1-1 Expressed in brush cells (Atoh1- Insm1-).
TS28: intestine epithelium Present 14_1-3 , 14_1-1 Expressed in brush cells (Atoh1- Insm1-).
Specimen 14 Villi-2: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: intestinal villus Present 14_2-1 , 14_2-3 Expressed in brush cells (Atoh1- Insm1-).
TS28: intestine epithelium Present 14_2-1 , 14_2-3 Expressed in brush cells (Atoh1- Insm1-).
Specimen 14 Crypts-1: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Weak 14_3-1 , 14_3-3 Expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
TS28: intestine epithelium Weak 14_3-1 , 14_3-3 Expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
Specimen 14 Crypts-2: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Present 14_4-3 , 14_4-1 Expressed in mature brush cells (Atoh1- Insm1-). Weakly expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
TS28: intestine epithelium Present 14_4-3 , 14_4-1 Expressed in mature brush cells (Atoh1- Insm1-). Weakly expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
Specimen 17A: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Dclk1; green - Gfi1b (assay MGI:6741116); orange - Insm1 (assay MGI:67411065).
Structure Level Pattern Image Note
TS26: intestinal villus Present Regionally restricted 17A-1 , 17A-4 Co-expressed with Gfi1b in brush cells in the intervillus trough.
TS26: intestine epithelium Present Regionally restricted 17A-1 , 17A-4 Co-expressed with Gfi1b in brush cells in the intervillus trough.
Specimen 17C: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Dclk1; green - Gfi1b (assay MGI:6741116); orange - Insm1 (assay MGI:67411065).
Structure Level Pattern Image Note
TS26: intestinal villus Present 17C-3 , 17C-1 Co-expressed with Gfi1b in relatively mature brush cells.
TS26: intestine epithelium Present 17C-3 , 17C-1 Co-expressed with Gfi1b in relatively mature brush cells.
Specimen 18 villi +/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Present Regionally restricted 18_upper left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
TS26: intestine epithelium Present Regionally restricted 18_upper left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
Specimen 18 villi -/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Absent 18_upper right
TS26: intestine epithelium Absent 18_upper right
Specimen 18 intervillus +/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Present Regionally restricted 18_lower left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
TS26: intestine epithelium Present Regionally restricted 18_lower left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
Specimen 18 intervillus -/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Absent 18_lower right
TS26: intestine epithelium Absent 18_lower right
Specimen not shown: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Structure Level Pattern Image Note
TS26: stomach epithelium Present Expressed in brush cells (Insm1-).
Specimen not shown: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Structure Level Pattern Image Note
TS26: stomach epithelium Present Expressed in brush cells (Insm1-).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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