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Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6741065
Gene symbol: Insm1
Gene name: insulinoma-associated 1
Antibody: Insm1 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 3A left: postnatal (more )
Note: Double labeled: orange - Insm1; red - lectin UEA-I.
Structure Level Pattern Image Note
TS28: intestinal villus Present 3A-5 , 3A-1 Expressed in enteroendocrine cells.
TS28: intestine epithelium Present 3A-5 , 3A-1 Expressed in enteroendocrine cells.
Specimen 3A right: postnatal (more )
Note: Double labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050).
Structure Level Pattern Image Note
TS28: intestinal villus Present 3A-2 , 3A-6 Expressed in enteroendocrine cells.
TS28: intestine epithelium Present 3A-2 , 3A-6 Expressed in enteroendocrine cells.
Specimen 3B left: postnatal (more )
Note: Double labeled: orange - Insm1; red - lectin UEA-I.
Structure Level Pattern Image Note
TS28: intestinal villus Present 3B-1 , 3B-5 Expressed in enteroendocrine cells.
TS28: intestine epithelium Present 3B-1 , 3B-5 Expressed in enteroendocrine cells.
Specimen 3B right: postnatal (more )
Note: Double labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050).
Structure Level Pattern Image Note
TS28: intestinal villus Present 3B-6 , 3B-2 Expressed in enteroendocrine cells.
TS28: intestine epithelium Present 3B-6 , 3B-2 Expressed in enteroendocrine cells.
Specimen 6: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: orange - Insm1; green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 6_1 , 6_5 Expressed in enteroendocrine cells (Gfi1b-).
TS28: intestine epithelium Present 6_1 , 6_5 Expressed in enteroendocrine cells (Gfi1b-).
Specimen 14 Villi-1: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: intestinal villus Present 14_1-4 , 14_1-1 No expression in the brush cells.
TS28: intestine epithelium Present 14_1-4 , 14_1-1 No expression in the brush cells.
Specimen 14 Villi-2: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: intestinal villus Present 14_2-1 , 14_2-4 Expressed in enteroendocrine cells.
TS28: intestine epithelium Present 14_2-1 , 14_2-4 Expressed in enteroendocrine cells.
Specimen 14 Crypts-1: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Present 14_3-1 , 14_3-4 No expression in the brush cells or Paneth cells.
TS28: intestine epithelium Present 14_3-1 , 14_3-4 No expression in the brush cells or Paneth cells.
Specimen 14 Crypts-2: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Present 14_4-1 , 14_4-4 Expressed in the enteroendocrine cells.
TS28: intestine epithelium Present 14_4-1 , 14_4-4 Expressed in the enteroendocrine cells.
Specimen 15A: postnatal; Atoh1tm1(cre)Gan/Atoh1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: orange - Insm1; green - beta-gal; red - lectin UEA-I.
Structure Level Pattern Image Note
TS28: intestinal villus Present 15A Insm1 , 15A merge Expressed in enteroendocrine cells.
TS28: intestine epithelium Present 15A Insm1 , 15A merge Expressed in enteroendocrine cells.
Specimen 15B: postnatal; Atoh1tm1(cre)Gan/Atoh1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: orange - Insm1; green - beta-gal; red - lectin UEA-I.
Structure Level Pattern Image Note
TS28: intestinal villus Present 15B merge , 15B Insm1 Expressed in enteroendocrine cells.
TS28: intestine epithelium Present 15B merge , 15B Insm1 Expressed in enteroendocrine cells.
Specimen 15C: postnatal; Atoh1tm1(cre)Gan/Atoh1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: orange - Insm1; green - beta-gal; red - lectin UEA-I.
Structure Level Pattern Image Note
TS28: intestinal villus Present 15C Insm1 , 15C merge Expressed in enteroendocrine cells. No expression in the brush cell.
TS28: intestine epithelium Present 15C Insm1 , 15C merge Expressed in enteroendocrine cells. No expression in the brush cell.
Specimen 17A: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Insm1; green - Gfi1b (assay MGI:6741116); red - Dclk1 (assay MGI:67411050).
Structure Level Pattern Image Note
TS26: intestinal villus Present 17A-5 , 17A-1 Expressed in enteroendocrine cells (Gfi1b- Dclk1-).
TS26: intestine epithelium Present 17A-5 , 17A-1 Expressed in enteroendocrine cells (Gfi1b- Dclk1-).
Specimen 17C: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Insm1; green - Gfi1b (assay MGI:6741116); red - Dclk1 (assay MGI:67411050).
Structure Level Pattern Image Note
TS26: intestinal villus Present 17C-1 , 17C-4 Expressed in enteroendocrine cells (Gfi1b- Dclk1-).
TS26: intestine epithelium Present 17C-1 , 17C-4 Expressed in enteroendocrine cells (Gfi1b- Dclk1-).
Specimen 18 villi +/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Note: Double labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050).
Structure Level Pattern Image Note
TS26: intestinal villus Present 18_upper left Expressed in enteroendocrine cells (Dclk1-).
TS26: intestine epithelium Present 18_upper left Expressed in enteroendocrine cells (Dclk1-).
Specimen 18 villi -/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Note: Double labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050).
Structure Level Pattern Image Note
TS26: intestinal villus Absent 18_upper right No enteroendocrine cells were observed.
TS26: intestine epithelium Absent 18_upper right No enteroendocrine cells were observed.
Specimen 18 intervillus +/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Note: Double labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050).
Structure Level Pattern Image Note
TS26: intestinal villus Present 18_lower left Expressed in enteroendocrine cells (Dclk1-).
TS26: intestine epithelium Present 18_lower left Expressed in enteroendocrine cells (Dclk1-).
Specimen 18 intervillus -/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Note: Double labeled: orange - Insm1; red - Dclk1 (assay MGI:6741050).
Structure Level Pattern Image Note
TS26: intestinal villus Absent 18_lower right No enteroendocrine cells were observed.
TS26: intestine epithelium Absent 18_lower right No enteroendocrine cells were observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory