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Gene Expression Data
Assay Details
Assay
Reference: J:293793 Basta JM, et al., The core SWI/SNF catalytic subunit Brg1 regulates nephron progenitor cell proliferation and differentiation. Dev Biol. 2020 Aug 15;464(2):176-187
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6740178
Gene symbol: Six2
Gene name: sine oculis-related homeobox 2
Antibody: anti-Six2 (11562-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3C Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: green - Six2; red - cytokeratin; blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 3C E14 Brg1flx/flx
Specimen 3A Six2Cre Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - cytokeratin; blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Weak 3C E14 Six2Cre Brg1flx/flx Number of expressing mesenchyme caps significantly diminshed.
Specimen 3C E16 Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: green - Six2; red - cytokeratin; blue - DAPI.
Structure Level Pattern Image Note
TS24: cap mesenchyme Present 3C E16 Brg1flx/flx
Specimen 3C E16 Six2Cre Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - cytokeratin; blue - DAPI.
Structure Level Pattern Image Note
TS24: cap mesenchyme Weak 3C E16 Six2Cre Brg1flx/flx Number of expressing mesenchyme caps significantly diminshed.
Specimen E17 Brg1flx/flx not shown: embryonic day 17.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Structure Level Pattern Image Note
TS25: cap mesenchyme Present
Specimen E17 Six2Cre Brg1flx/flx not shown: embryonic day 17.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: cap mesenchyme Weak Number of expressing mesenchyme caps significantly diminished.
Specimen 4A E14 Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: green - Six2; red - phospho-histone H3; blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 4A E14 Brg1flx/flx
Specimen 4A E14 Six2Cre Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - phospho-histone H3; blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Weak Regionally restricted 4A E14 Six2Cre Brg1flx/flx Reduced number of Six2/pHH3 double-positive cells.
Specimen 4A E16 Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: green - Six2; red - phospho-histone H3; blue - DAPI.
Structure Level Pattern Image Note
TS24: cap mesenchyme Present 4A E16 Brg1flx/flx
Specimen 4A E16 Six2Cre Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - phospho-histone H3; blue - DAPI.
Structure Level Pattern Image Note
TS24: cap mesenchyme Weak Regionally restricted 4A E16 Six2Cre Brg1flx/flx Reduced number of Six2/pHH3 double-positive cells.
Specimen 4D E14 Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: green - Six2; red - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 4D E14 Brg1flx/flx
Specimen 4D E14 Six2Cre Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 4D E14 Six2Cre Brg1flx/flx Authors report no significant increase in apoptosis in nephron progenitors.
Specimen 4D E16 Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: green - Six2; red - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS24: cap mesenchyme Present 4D E16 Brg1flx/flx
Specimen 4D E16 Six2Cre Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - TUNEL; blue - DAPI.
Structure Level Pattern Image Note
TS24: cap mesenchyme Present 4D E16 Six2Cre Brg1flx/flx Authors report no significant increase in apoptosis in nephron progenitors.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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