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Gene Expression Data
Assay Details
Assay
Reference: J:185870 Hazen VM, et al., BMP receptor-activated Smads confer diverse functions during the development of the dorsal spinal cord. Dev Biol. 2012 Jul 15;367(2):216-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6727114
Gene symbol: Lhx9
Gene name: LIM homeobox protein 9
Antibody: anti-LH2A/B
Detection system: Secondary antibody coupled to fluorescein
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 4A/C: embryonic day 10.5; Smad1tm1Abr/Smad1tm1Abr (more )
Note: Double labeled: green - Lhx2 and Lhx9; red - Isl1 (assay MGI:6727048).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 4C , 4A Expressed in commissural (dI1) neurons.

Specimen 4E/G: embryonic day 10.5; Smad1tm1Abr/Smad1tm1Abr, Tg(Pou3f4-cre)32Cren/0 (more )
Note: Conditional mutant. Double labeled: green - Lhx2 and Lhx9; red - Isl1 (assay MGI:6727048).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 4E , 4G Expressed in commissural (dI1) neurons, similar as in the control.

Specimen 4I/K: embryonic day 10.5; Smad5tm1Huy/Smad5tm1Huy (more )
Note: Double labeled: green - Lhx2 and Lhx9; red - Isl1 (assay MGI:6727048).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 4I , 4K Expressed in commissural (dI1) neurons.

Specimen 4M/O: embryonic day 10.5; Smad5tm1Huy/Smad5tm1Huy, Tg(Pou3f4-cre)32Cren/0 (more )
Note: Conditional mutant. Double labeled: green - Lhx2 and Lhx9; red - Isl1 (assay MGI:6727048).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 4O , 4M Reduced expression (>20%) in commissural (dI1) neurons, compared with the control.

Specimen not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted Expressed in commissural (dI1) neurons.

Specimen not shown: embryonic day 10.5; Smad1tm1Abr/Smad1tm1Abr, Smad5tm1Huy/Smad5tm1Huy, Tg(Atoh1-cre)1Bfri/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted Reduced expression (30%) in commissural (dI1) neurons, compared with the control.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory