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Gene Expression Data
Assay Details
Assay
Reference: J:228503 Altieri SC, et al., En1 directs superior olivary complex neuron positioning, survival, and expression of FoxP1. Dev Biol. 2015 Dec 1;408(1):99-108
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6725812
Gene symbol: Chat
Gene name: choline O-acetyltransferase
Antibody: anti-Chat
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2F'/F": postnatal adult; En1tm2(cre)Wrst/En1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Chat; red - Cre reporter.
Structure Level Pattern Image Note
TS28: nucleus of trapezoid body Present 2F'/F" Co-expressed with En1-cre in neurons in the vental nuclei.
Specimen 2J'/J": postnatal adult; En1tm2(cre)Wrst/En1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Chat; red - Cre reporter.
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted 2J'/J" Expressed in the lateral olivocochlear bundle neurons that reside within the superior olivary complex.
Specimen 2K'/K": postnatal adult; En1tm2(cre)Wrst/En1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Chat; red - Cre reporter.
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted 2K'/K" Expressed in the medial olivocochlear bundle neurons that reside within the superior olivary complex.
Specimen 3E: postnatal adult (more )
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted 3E Expressed in the lateral olivocochlear bundle neurons.
Specimen 3F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted 3F Expressed in the medial olivocochlear bundle neurons.
TS28: nucleus of trapezoid body Present Regionally restricted 3F Expressed in the cholinergic neurons in the ventral nuclei.
Specimen 3G: postnatal adult; Egr2tm2(cre)Pch/Egr2+, En1tm8.1Alj/En1tm8.1Alj (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted 3G Expressed in the lateral olivocochlear bundle neurons, similar as in the control.
Specimen 3H: postnatal adult; Egr2tm2(cre)Pch/Egr2+, En1tm8.1Alj/En1tm8.1Alj (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted 3H Expressed in the medial olivocochlear bundle neurons, similar as in the control.
TS28: nucleus of trapezoid body Ambiguous 3H No expression in the cholinergic neurons in the ventral nuclei.
Specimen S1A'/A": postnatal adult; En1tm2(cre)Wrst/En1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Chat; red - Glycine.
Structure Level Pattern Image Note
TS28: nucleus of trapezoid body Present Regionally restricted S1A'/A" Expressed in the vental nuclei.
Specimen S1B'/B": postnatal adult; En1tm2(cre)Wrst/En1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Chat; red - Glycine.
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted S1B'/B" Expressed in the lateral olivocochlear bundle neurons.
Specimen S1C'/C": postnatal adult; En1tm2(cre)Wrst/En1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Chat; red - Glycine.
Structure Level Pattern Image Note
TS28: brainstem nucleus Present Regionally restricted S1C'/C" Expressed in the medial olivocochlear bundle neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory