Ccn2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:117074 Pummila M, et al., Ectodysplasin has a dual role in ectodermal organogenesis: inhibition of Bmp activity and induction of Shh expression. Development. 2007 Jan;134(1):117-25
Assay type:	RNA in situ
MGI Accession ID:	MGI:6724520
Gene symbol:	Ccn2
Gene name:	cellular communication network factor 2

Probe:	Ctgf cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5A ©

Specimen 5A: embryonic day 14.5 (more

)

Structure	Level	Image	Note
TS22: hair epidermal placode	Present	5A	Expression is often concentrated at the circumference of the placode.
TS22: phalanx cartilage condensation	Present	5A
TS22: mystacial pad	Absent	5A

5B ©

Specimen 5B: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hair epidermal placode	Present		5B	Expression is often concentrated at the circumference of the placode.

5C ©

5C' ©

Specimen 5C: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hair epidermal placode	Present		5C' , 5C	Expression is often concentrated at the circumference of the placode.

5D ©

Specimen 5D: embryonic day 14.5; Eda^Ta/Eda^Ta (more

)

Structure	Level	Pattern	Image	Note
TS22: skin	Absent		5D

5H ©

Specimen 5H: embryonic day 14.5 (more

)
Note: Vibratome section.

Structure	Level	Pattern	Image	Note
TS22: hair epidermal placode	Present		5H

5K ©

Specimen 5K: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present		5K	Expression is in molar and incisor placodes.
TS20: Meckel's cartilage	Strong		5K

5M ©

Specimen 5M: embryonic day 12.5; Eda^Ta/Eda^Ta (more

)

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present		5M	Expression in this mutant is the same as in wild-type.
TS20: Meckel's cartilage	Strong		5M

Specimen not shown E14-/-: embryonic day 14.5; Eda^Ta/Eda^Ta (more

)

Structure	Level	Pattern	Image	Note
TS22: phalanx cartilage condensation	Present			Expression in this mutant is the same as in wild-type embbryos.

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