About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:278880 Li Y, et al., Embryonic senescent cells re-enter cell cycle and contribute to tissues after birth. Cell Res. 2018 Jul;28(7):775-778
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6724452
Gene symbol: Cdkn1a
Gene name: cyclin dependent kinase inhibitor 1A
Reporter: ESR
Antibody: anti-ESR (ab27595)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 1f - E10.5: embryonic day 10.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present Single cells 1f ESR E10.5 Expression is seen in senescent cells.
Specimen 1f - E11.5: embryonic day 11.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS19: handplate Present Single cells 1f ESR E11.5 Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen 1f - E12.5: embryonic day 12.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS20: hand Present Single cells 1f ESR E12.5 Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen 1f - E13.5: embryonic day 13.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS21: hand Present Single cells 1f ESR E13.5 Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen 1f - E14.5: embryonic day 14.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS22: hand Present Single cells 1f ESR E14.5 Expression is seen in senescent cells in the apical ectodermal ridge; their frequency is decreasing.
Specimen 1f - E15.5: embryonic day 15.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS23: hand Absent 1f ESR E15.5 No senescent cells are seen.
Specimen S2c: embryonic day 12.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Note: Triple labeled: red - Cdkn1a reporter; green - Cdkn1a protein (assay MGI:6724451); blue - DAPI. Also stained for endogenous B-galactosidase to reveal senescent cells.
Structure Level Pattern Image Note
TS20: hand Present Single cells S2c ESR/P21 , S2c ESR Co-labeling with B-galactosidase confirms expression is seen in senescent cells.
Specimen S3a: embryonic day 12.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Note: Triple labeled: red - Cdkn1a reporter; green - Cd44 (assay MGI:6724476); blue - DAPI.
Structure Level Pattern Image Note
TS20: hand Present Single cells S3a upper red , S3a lower merge , S3a upper merge , S3a lower red Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen S3b: embryonic day 12.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Note: Triple labeled: red - Cdkn1a reporter; green - Cbx3 (assay MGI:6724477); blue - DAPI.
Structure Level Pattern Image Note
TS20: hand Present Single cells S3b upper merge , S3b lower red , S3b upper red , S3b lower merge Expression is seen in senescent cells in the apical ectodermal ridge.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory