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Gene Expression Data
Assay Details
Assay
Reference: J:278880 Li Y, et al., Embryonic senescent cells re-enter cell cycle and contribute to tissues after birth. Cell Res. 2018 Jul;28(7):775-778
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6724451
Gene symbol: Cdkn1a
Gene name: cyclin dependent kinase inhibitor 1A
Antibody: anti-p21 (HUGO-291; ab107099)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1f - E10.5: embryonic day 10.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present Single cells 1f P21 E10.5 Expression is seen in senescent cells.
Specimen 1f - E11.5: embryonic day 11.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS19: handplate Present Single cells 1f P21 E11.5 Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen 1f - E12.5: embryonic day 12.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS20: hand Present Single cells 1f P21 E12.5 Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen 1f - E13.5: embryonic day 13.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS21: hand Present Single cells 1f P21 E13.5 Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen 1f - E14.5: embryonic day 14.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS22: hand Present Single cells 1f P21 E14.5 Expression is seen in senescent cells in the apical ectodermal ridge; their frequency is decreasing.
Specimen 1f - E15.5: embryonic day 15.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Structure Level Pattern Image Note
TS23: hand Absent 1f P21 E15.5 No senescent cells are seen.
Specimen S2c: embryonic day 12.5; Cdkn1aem3(cre/ERT)Smoc/Cdkn1a+ (more )
Note: Triple labeled: green - Cdkn1a protein; red - Cdkn1a reporter (assay MGI:6724452); blue - DAPI. Also stained for endogenous B-galactosidase to reveal senescent cells.
Structure Level Pattern Image Note
TS20: hand Present Single cells S2c P21 , S2c ESR/P21 Co-labeling with B-galactosidase confirms expression is seen in senescent cells.
Specimen S2e - E11.5: embryonic day 11.5; Cdkn1aem1Smoc/Cdkn1a+ (more )
Note: Triple labeled: green - Cdkn1a protein; red - Cdkn1a reporter (assay MGI:6724471); blue - DAPI.
Structure Level Pattern Image Note
TS19: handplate Present Single cells S2e upper green , S2e upper merge Expression is seen in senescent cells in the apical ectodermal ridge.
Specimen S2e - E12.5: embryonic day 12.5; Cdkn1aem1Smoc/Cdkn1a+ (more )
Note: Triple labeled: green - Cdkn1a protein; red - Cdkn1a reporter (assay MGI:6724471); blue - DAPI.
Structure Level Pattern Image Note
TS20: hand Present Single cells S2e lower green , S2e lower merge Expression is seen in senescent cells in the apical ectodermal ridge.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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