About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:306370 Harland LTG, et al., The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nat Cell Biol. 2021 Jan;23(1):61-74
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6721392
Gene symbol: Eomes
Gene name: eomesodermin
Antibody: anti-Eomes/Tbr2 (ab23345)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1b: embryonic day 6.5 (more )
Note: Early-streak stage embryo. Double labeled: green - Eomes; red - Brachyury (assay MGI:6721393).
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1b green , 1b merge Expression is seen in proximal epiblast/primitive streak cells.
TS9: primitive streak Present 1b merge , 1b green Expression is seen in proximal epiblast/primitive streak cells.
Specimen 1c: embryonic day 6.5 (more )
Note: Mid-streak stage embryo. Double labeled: green - Eomes; red - Brachyury (assay MGI:6721393).
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 1c green , 1c merge Expression is seen in proximal epiblast/primitive streak cells.
TS10: primitive streak Present 1c merge , 1c green Expression is seen in proximal epiblast/primitive streak cells.
Specimen 1f: embryonic day 7.5; Eomestm2.1Rob/Eomes+ (more )
Note: Fixed in 1% paraformaldehyde. Double labeled: red - Eomes protein; green - Eomes reporter (assay MGI:6721395).
Structure Level Pattern Image Note
TS11: extraembryonic mesoderm Absent 1f merge , 1f red , 1f red inset , 1f merge inset Reporter expression persists in this tissue longer than the endogenous protein.
Specimen ED1a: embryonic day 6.5 (more )
Note: Mid-streak stage embryo. Double labeled: green - Eomes; red - Kdr (assay MGI:6721396).
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Present Extended Data 1a merge , Extended Data 1a green
Specimen ED1b: embryonic day 7.5 (more )
Note: Late-streak stage embryo. Fixed in 1% paraformaldehyde. Double labeled: green - Eomes; red - Kdr (assay MGI:6721396).
Structure Level Pattern Image Note
TS11: yolk sac Absent Extended Data 1b merge inset , Extended Data 1b green , Extended Data 1b green inset , Extended Data 1b merge
TS11: embryo mesoderm Present Extended Data 1b merge inset , Extended Data 1b merge , Extended Data 1b green inset , Extended Data 1b green
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory