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Gene Expression Data
Assay Details
Assay
Reference: J:302432 Nasirova N, et al., Mapping Cell Types and Efferent Pathways in the Ascending Relaxin-3 System of the Nucleus Incertus. eNeuro. 2020 Nov/Dec;7(6):ENEURO.0272-20.2020
Assay type: RNA in situ
MGI Accession ID: MGI:6719268
Gene symbol: Rln3
Gene name: relaxin 3
Probe: Mm-Rln3 (459921)
Probe preparation: Antisense RNA
Visualized with: Fluorescein
Assay notes: RNAscope assay.
Results
Specimen 1D: postnatal; Rln3tm1.1(cre/GFP)Et/Rln3+ (more )
Note: Double labeled: red - Rln3 RNA; green - Rln3 reporter (assay MGI:6718943)
Structure Level Pattern Image Note
TS28: brain gray matter Present Regionally restricted 1D Expression is seen in a small fraction of neurons in the central pontine gray.
Specimen 1F: postnatal; Rln3tm1.1(cre/GFP)Et/Rln3+ (more )
Note: Double labeled: red - Rln3 RNA; green - Rln3 reporter (assay MGI:6718943)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 1F , 1F merge , 1F Rln3 Expressing neurons are seen in the central nucleus incertus.
Specimen 1H: postnatal; Rln3tm1.1(cre/GFP)Et/Rln3+ (more )
Note: Double labeled: red - Rln3 RNA; green - Rln3 reporter (assay MGI:6718943)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 1H Expressing neurons are seen in the caudal nucleus incertus.
Specimen 1K: postnatal (more )
Note: Double labeled: green - Rln3; red - Gad2 (assay MGI:6719374)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 1K3 , 1K1 Expressing neurons are seen in the nucleus incertus.
TS28: brain gray matter Present Regionally restricted 1K1 , 1K3 Expression is seen in a small fraction of neurons in the central pontine gray.
Specimen 3A: postnatal (more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: midbrain tegmentum Present Single cells 3A
Specimen 3B: postnatal (more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 3B merge , 3B green , 3B Expressing neurons are seen in the rostral nucleus incertus.
Specimen 3C: postnatal (more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 3C merge , 3C , 3C green Expressing neurons are seen in the central nucleus incertus.
Specimen 3D: postnatal (more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Single cells 3D , 3D green , 3D merge Expressing neurons are seen in the caudal nucleus incertus.
Specimen 3E: postnatal (more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: brain gray matter Present Single cells 3E green , 3E , 3E merge Expressing neurons are seen in the central pontine gray.
Specimen 3F: postnatal (more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: dorsal tegmental nucleus Absent 3F No expression is seen in the posterodorsal tegmental nucleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory