Antibody:	anti-LHX3 (ab14555)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS22: superior semicircular canal epithelium	Present	Regionally restricted	5a WT E14.5 upper	Expression is seen in the hair cells of the anterior crista.
TS22: lateral semicircular canal epithelium	Present	Regionally restricted	5a WT E14.5 upper	Expression is seen in the hair cells of the lateral crista.

Structure	Level	Pattern	Image	Note
TS22: utricle epithelium	Present	Regionally restricted	5a WT E14.5 lower left	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Absent		5a WT E13.5 upper	Hair cells have not differentiated at this age.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Absent		5a WT E14.5 lower right	Hair cells have not differentiated at this age.

Structure	Level	Pattern	Image	Note
TS21: superior semicircular canal epithelium	Present	Regionally restricted	5a hom E13.5 upper left	Expression is seen in the hair cells of the anterior crista; no differences in expression were observed between wild type and the mutants.
TS21: lateral semicircular canal epithelium	Present	Regionally restricted	5a hom E13.5 upper left	Expression is seen in the hair cells of the lateral crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS21: saccule epithelium	Present	Regionally restricted	5a hom E13.5 lower left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS21: utricle epithelium	Present	Regionally restricted	5a hom E13.5 lower center	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS21: posterior semicircular canal epithelium	Present	Regionally restricted	5a hom E13.5 upper right	Expression is seen in the hair cells of the posterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Absent		5a hom E13.5 lower right	As in wild type, hair cells have not differentiated at this age.

Structure	Level	Pattern	Image	Note
TS22: saccule epithelium	Present	Regionally restricted	5a hom E14.5 left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.
TS22: utricle epithelium	Present	Regionally restricted	5a hom E14.5 left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS22: lateral semicircular canal epithelium	Present	Regionally restricted	5a hom E14.5 upper right	Expression is seen in the hair cells of the lateral crista; no differences in expression were observed between wild type and the mutants.
TS22: superior semicircular canal epithelium	Present	Regionally restricted	5a hom E14.5 upper right	Expression is seen in the hair cells of the anterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Absent		5a hom E14.5 lower right	As in wild type, hair cells have not differentiated at this age.

Structure	Level	Pattern	Image	Note
TS23: saccule epithelium	Present	Regionally restricted	5a WT E15.5 left	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS23: posterior semicircular canal epithelium	Present	Regionally restricted	5a WT E15.5 right	Expression is seen in the hair cells of the posterior crista.

Structure	Level	Pattern	Image	Note
TS25: utricle epithelium	Present	Regionally restricted	5a WT E17.5 left	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present	Regionally restricted	5a WT E17.5 right	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS23: utricle epithelium	Present	Regionally restricted	5a hom E15.5 upper left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Regionally restricted	5a hom E15.5 lower left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS23: posterior semicircular canal epithelium	Present	Regionally restricted	5a hom E15.5 right	Expression is seen in the hair cells of the posterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: lateral semicircular canal epithelium	Present	Regionally restricted	5a hom E17.5 upper left	Expression is seen in the hair cells of the lateral crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: superior semicircular canal epithelium	Present	Regionally restricted	5a hom E17.5 upper center	Expression is seen in the hair cells of the anterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: utricle epithelium	Present	Regionally restricted	5a hom E17.5 upper right	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: utricle epithelium	Present	Regionally restricted	5a hom E17.5 lower left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present	Regionally restricted	5a hom E17.5 lower right	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.