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Gene Expression Data
Assay Details
Assay
Reference: J:297267 Tang Q, et al., Gfi1-GCE inducible Cre line for hair cell-specific gene manipulation in mouse inner ear. Genesis. 2019 Sep;57(9):e23304
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6717266
Gene symbol: Gfi1
Gene name: growth factor independent 1 transcription repressor
Reporter: GFP
Antibody: anti-GFP (ab13970)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1c: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 1c GFP , 1c merge Without tamoxifen, expression was seen in the cytoplasm of the inner and outer hair cells.
Specimen 1e: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Multi-labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 1e GFP , 1e merge Without tamoxifen, expression was seen in the cytoplasm of the inner and outer hair cells.
Specimen 2a - u/s: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS21: saccule epithelium Present Regionally restricted 2a left Expression is seen in the hair cells.
TS21: utricle epithelium Present Regionally restricted 2a left Expression is seen in the hair cells.
Specimen 2a - ac: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS21: superior semicircular canal epithelium Present Regionally restricted 2a upper right Expression is seen in the hair cells of the anterior crista.
Specimen 2a - pc: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS21: posterior semicircular canal epithelium Present Regionally restricted 2a lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2b - u/s: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS22: saccule epithelium Present Regionally restricted 2b left Expression is seen in the hair cells.
TS22: utricle epithelium Present Regionally restricted 2b left Expression is seen in the hair cells.
Specimen 2b - ac/lc: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 2b upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS22: superior semicircular canal epithelium Present Regionally restricted 2b upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2b - pc: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS22: posterior semicircular canal epithelium Present Regionally restricted 2b lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2c - s: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS23: saccule epithelium Present Regionally restricted 2c upper left Expression is seen in the hair cells.
Specimen 2c - u: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS23: utricle epithelium Present Regionally restricted 2c lower left Expression is seen in the hair cells.
Specimen 2c - ac: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS23: superior semicircular canal epithelium Present Regionally restricted 2c upper center Expression is seen in the hair cells of the anterior crista.
Specimen 2c - lc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS23: lateral semicircular canal epithelium Present Regionally restricted 2c upper right Expression is seen in the hair cells of the lateral crista.
Specimen 2c - pc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS23: posterior semicircular canal epithelium Present Regionally restricted 2c lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2d - s: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS24: saccule epithelium Present Regionally restricted 2d upper left Expression is seen in the hair cells.
Specimen 2d - u: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS24: utricle epithelium Present Regionally restricted 2d lower left Expression is seen in the hair cells.
Specimen 2d - ac/lc: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS24: lateral semicircular canal epithelium Present Regionally restricted 2d upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS24: superior semicircular canal epithelium Present Regionally restricted 2d upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2d - pc: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS24: posterior semicircular canal epithelium Present Regionally restricted 2d lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2e - s: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS25: saccule epithelium Present Regionally restricted 2e upper left Expression is seen in the hair cells.
Specimen 2e - u: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS25: utricle epithelium Present Regionally restricted 2e lower left Expression is seen in the hair cells.
Specimen 2e - ac/lc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS25: lateral semicircular canal epithelium Present Regionally restricted 2e upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS25: superior semicircular canal epithelium Present Regionally restricted 2e upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2e - pc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS25: posterior semicircular canal epithelium Present Regionally restricted 2e lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2f - s: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS27: saccule epithelium Present Regionally restricted 2f upper left Expression is seen in the hair cells.
Specimen 2f - u: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS27: utricle epithelium Present Regionally restricted 2f lower left Expression is seen in the hair cells.
Specimen 2f - lc: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS27: lateral semicircular canal Present Regionally restricted 2f upper right Expression is seen in the hair cells of the lateral crista.
Specimen 2f - pc: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); blue - DAPI.
Structure Level Pattern Image Note
TS27: posterior semicircular canal Present Regionally restricted 2f lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2g: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS22: cochlea Absent 2g GFP , 2g Merge No hair cells were seen in the cochlea at this age.
Specimen 2h: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS23: cochlea Present Regionally restricted 2h GFP , 2h Merge Expression is seen in the hair cells of the basal cochlea. (As hair cell differentiation proceeds, expression will expand to the middle and then apical cochlea.)
Specimen 2i: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 2i GFP , 2i Merge Expression is seen in the inner and outer hair cells.
Specimen 2j: postnatal day 7; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Double labeled: green - Gfi1 reporter; red - Gfi1 protein (assay MGI:6717267).
Structure Level Pattern Image Note
TS28: cochlea Present Regionally restricted 2j left , 2j right Expression is seen in the inner and outer hair cells.
Specimen 5a - hom - E13.5 - ac/lc: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS21: superior semicircular canal epithelium Present Regionally restricted 5a hom E13.5 upper left Expression is seen in the hair cells of the anterior crista.
TS21: lateral semicircular canal epithelium Present Regionally restricted 5a hom E13.5 upper left Expression is seen in the hair cells of the lateral crista.
Specimen 5a - hom - E13.5 - s: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS21: saccule epithelium Present Regionally restricted 5a hom E13.5 lower left Expression is seen in the hair cells.
Specimen 5a - hom - E13.5 - u: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS21: utricle epithelium Present Regionally restricted 5a hom E13.5 lower center Expression is seen in the hair cells.
Specimen 5a - hom - E13.5 - pc: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS21: posterior semicircular canal epithelium Present Regionally restricted 5a hom E13.5 upper right Expression is seen in the hair cells of the posterior crista.
Specimen 5a - hom - E13.5 - cd: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS21: cochlear duct Absent 5a hom E13.5 lower right Hair cells have not differentiated at this age.
Specimen 5a - hom - E14.5 - s/u: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS22: saccule epithelium Present Regionally restricted 5a hom E14.5 left Expression is seen in the hair cells.
TS22: utricle epithelium Present Regionally restricted 5a hom E14.5 left Expression is seen in the hair cells.
Specimen 5a - hom - E14.5 - lc/ac: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 5a hom E14.5 upper right Expression is seen in the hair cells of the lateral crista.
TS22: superior semicircular canal epithelium Present Regionally restricted 5a hom E14.5 upper right Expression is seen in the hair cells of the anterior crista.
Specimen 5a - hom - E14.5 - cd: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS22: cochlear duct Absent 5a hom E14.5 lower right Hair cells have not differentiated at this age.
Specimen 5a - hom - E15.5 - u: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS23: utricle epithelium Present Regionally restricted 5a hom E15.5 upper left Expression is seen in the hair cells.
Specimen 5a - hom - E15.5 - cd: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS23: cochlear duct epithelium Present Regionally restricted 5a hom E15.5 lower left Expression is seen in the hair cells.
Specimen 5a - hom - E15.5 - pc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS23: posterior semicircular canal epithelium Present Regionally restricted 5a hom E15.5 right Expression is seen in the hair cells of the posterior crista.
Specimen 5a - hom - E17.5 - lc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS25: lateral semicircular canal epithelium Present Regionally restricted 5a hom E17.5 upper left Expression is seen in the hair cells of the lateral crista.
Specimen 5a - hom - E17.5 - ac: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS25: superior semicircular canal epithelium Present Regionally restricted 5a hom E17.5 upper center Expression is seen in the hair cells of the anterior crista.
Specimen 5a - hom - E17.5 - u (upper): embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS25: utricle epithelium Present Regionally restricted 5a hom E17.5 upper right Expression is seen in the hair cells.
Specimen 5a - hom - E17.5 - u (lower): embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS25: utricle epithelium Present Regionally restricted 5a hom E17.5 lower left Expression is seen in the hair cells.
Specimen 5a - hom - E17.5 - cd: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Lhx3 (assay MGI:6717272); blue - DAPI.
Structure Level Pattern Image Note
TS25: cochlear duct epithelium Present Regionally restricted 5a hom E17.5 lower right Expression is seen in the hair cells.
Specimen 5b - hom - E13.5 - u: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS21: utricle epithelium Present Regionally restricted 5b hom E13.5 upper left Expression is seen in the hair cells.
Specimen 5b - hom - E13.5 - s: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS21: saccule epithelium Present Regionally restricted 5b hom E13.5 lower left Expression is seen in the hair cells.
Specimen 5b - hom - E13.5 - pc: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS21: posterior semicircular canal epithelium Present Regionally restricted 5b hom E13.5 upper right Expression is seen in the hair cells of the posterior crista.
Specimen 5b - hom - E13.5 - cd: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS21: cochlear duct Absent 5b hom E13.5 lower right Hair cells have not differentiated at this age.
Specimen 5b - hom - E14.5 - lc/ac: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 5b hom E14.5 upper left Expression is seen in the hair cells of the lateral crista.
TS22: superior semicircular canal epithelium Present Regionally restricted 5b hom E14.5 upper left Expression is seen in the hair cells of the anterior crista.
Specimen 5b - hom - E14.5 - u: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS22: utricle epithelium Present Regionally restricted 5b hom E14.5 lower left Expression is seen in the hair cells.
Specimen 5b - hom - E14.5 - s: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS22: saccule epithelium Present Regionally restricted 5b hom E14.5 upper right Expression is seen in the hair cells.
Specimen 5b - hom - E14.5 - cd: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS22: cochlear duct Absent 5b hom E14.5 lower right Hair cells have not differentiated at this age.
Specimen 5b - hom - E15.5 - lc/ac: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS23: lateral semicircular canal epithelium Present Regionally restricted 5b hom E15.5 upper left Expression is seen in the hair cells of the lateral crista.
TS23: superior semicircular canal epithelium Present Regionally restricted 5b hom E15.5 upper left Expression is seen in the hair cells of the anterior crista.
Specimen 5b - hom - E15.5 - pc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS23: posterior semicircular canal epithelium Present Regionally restricted 5b hom E15.5 upper right Expression is seen in the hair cells of the posterior crista.
Specimen 5b - hom - E15.5 - u: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS23: utricle epithelium Present Regionally restricted 5b hom E15.5 lower left Expression is seen in the hair cells.
Specimen 5b - hom - E15.5 - cd: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS23: cochlear duct epithelium Present Regionally restricted 5b hom E15.5 lower right Expression is seen in the hair cells.
Specimen 5b - hom - E17.5 - lc/ac: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS25: lateral semicircular canal epithelium Present Regionally restricted 5b hom E17.5 upper left Expression is seen in the hair cells of the lateral crista.
TS25: superior semicircular canal epithelium Present Regionally restricted 5b hom E17.5 upper left Expression is seen in the hair cells of the anterior crista.
Specimen 5b - hom - E17.5 - pc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS25: posterior semicircular canal epithelium Present Regionally restricted 5b hom E17.5 upper right Expression is seen in the hair cells of the posterior crista.
Specimen 5b - hom - E17.5 - u: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS25: utricle epithelium Present Regionally restricted 5b hom E17.5 lower left Expression is seen in the hair cells.
Specimen 5b - hom - E17.5 - s: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS25: saccule epithelium Present Regionally restricted 5b hom E17.5 lower center Expression is seen in the hair cells.
Specimen 5b - hom - E17.5 - cd: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Myo6 (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS25: cochlear duct epithelium Present Regionally restricted 5b hom E17.5 lower right Expression is seen in the hair cells.
Specimen 5c - hom - E13.5 - u: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS21: utricle epithelium Present Regionally restricted 5c hom E13.5 upper left Expression is seen in the hair cells.
Specimen 5c - hom - E13.5 - cd: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS21: cochlear duct Absent 5c hom E13.5 lower left Hair cells have not differentiated at this age.
Specimen 5c - hom - E13.5 - ac/lc: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS21: superior semicircular canal epithelium Present Regionally restricted 5c hom E13.5 right Expression is seen in the hair cells of the anterior crista.
TS21: lateral semicircular canal epithelium Present Regionally restricted 5c hom E13.5 right Expression is seen in the hair cells of the lateral crista.
Specimen 5c - hom - E14.5 - lc/ac: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 5c hom E14.5 upper left Expression is seen in the hair cells of the lateral crista.
TS22: superior semicircular canal epithelium Present Regionally restricted 5c hom E14.5 upper left Expression is seen in the hair cells of the anterior crista.
Specimen 5c - hom - E14.5 - u: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS22: utricle epithelium Present Regionally restricted 5c hom E14.5 lower left Expression is seen in the hair cells.
Specimen 5c - hom - E14.5 - cd: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS22: cochlear duct Absent 5c hom E14.5 right Hair cells have not differentiated at this age.
Specimen 5c - hom - E15.5 - lc/ac: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS23: lateral semicircular canal epithelium Present Regionally restricted 5c hom E15.5 upper left Expression is seen in the hair cells of the lateral crista.
TS23: superior semicircular canal epithelium Present Regionally restricted 5c hom E15.5 upper left Expression is seen in the hair cells of the anterior crista.
Specimen 5c - hom - E15.5 - s: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS23: saccule epithelium Present Regionally restricted 5c hom E15.5 lower left Expression is seen in the hair cells.
Specimen 5c - hom - E15.5 - u: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS23: utricle epithelium Present Regionally restricted 5c hom E15.5 lower center Expression is seen in the hair cells.
Specimen 5c - hom - E15.5 - cd: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS23: cochlear duct epithelium Present Regionally restricted 5c hom E15.5 right Expression is seen in the hair cells.
Specimen 5c - hom - E17.5 - u/s: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS25: utricle epithelium Present Regionally restricted 5c hom E17.5 left Expression is seen in the hair cells.
TS25: saccule epithelium Present Regionally restricted 5c hom E17.5 left Expression is seen in the hair cells.
Specimen 5c - hom - E17.5 - cd: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Triple labeled: green - Gfi1 reporter; red - Sox2 (assay MGI:6717273); blue - DAPI.
Structure Level Pattern Image Note
TS25: cochlear duct epithelium Present Regionally restricted 5c hom E17.5 right Expression is seen in the hair cells.
Specimen 6b - Apex: postnatal day 56; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Double labeled: green - Gfi1 reporter; gray - Myo6 (assay MGI:6717268).
Structure Level Pattern Image Note
TS28: cochlear duct epithelium Present Regionally restricted 6b upper left , 6b Apex Merge , 6b Apex GFP Expression is seen in the inner and outer cells of the apical cochlear duct.
Specimen 6b - Middle: postnatal day 56; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Double labeled: green - Gfi1 reporter; gray - Myo6 (assay MGI:6717268).
Structure Level Pattern Image Note
TS28: cochlear duct epithelium Present Regionally restricted 6b Middle GFP , 6b Middle Merge , 6b upper center Expression is seen in the inner and outer cells of the middle cochlear duct.
Specimen 6b - Basal: postnatal day 56; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Double labeled: green - Gfi1 reporter; gray - Myo6 (assay MGI:6717268).
Structure Level Pattern Image Note
TS28: cochlear duct epithelium Present Regionally restricted 6b upper right , 6b Basal Merge , 6b Basal GFP Expression is seen in the inner and outer cells of the basal cochlear duct.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory