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Gene Expression Data
Assay Details
Assay
Reference: J:297267 Tang Q, et al., Gfi1-GCE inducible Cre line for hair cell-specific gene manipulation in mouse inner ear. Genesis. 2019 Sep;57(9):e23304
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6717267
Gene symbol: Gfi1
Gene name: growth factor independent 1 transcription repressor
Antibody: anti-Gfi1 (N-20; sc-8558)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1c: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 1c merge , 1c GFI1 Expression is in the nuclei of the inner and outer hair cells.
Specimen 1e: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 1e merge , 1e GFI1 Expression is in the nuclei of the inner and outer hair cells.
Specimen 2a - u/s: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS21: saccule epithelium Present Regionally restricted 2a left Expression is seen in the hair cells.
TS21: utricle epithelium Present Regionally restricted 2a left Expression is seen in the hair cells.
Specimen 2a - ac: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS21: superior semicircular canal epithelium Present Regionally restricted 2a upper right Expression is seen in the hair cells of the anterior crista.
Specimen 2a - pc: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS21: posterior semicircular canal epithelium Present Regionally restricted 2a lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2b - u/s: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS22: saccule epithelium Present Regionally restricted 2b left Expression is seen in the hair cells.
TS22: utricle epithelium Present Regionally restricted 2b left Expression is seen in the hair cells.
Specimen 2b - ac/lc: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 2b upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS22: superior semicircular canal epithelium Present Regionally restricted 2b upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2b - pc: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS22: posterior semicircular canal epithelium Present Regionally restricted 2b lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2c - s: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: saccule epithelium Present Regionally restricted 2c upper left Expression is seen in the hair cells.
Specimen 2c - u: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: utricle epithelium Present Regionally restricted 2c lower left Expression is seen in the hair cells.
Specimen 2c - ac: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: superior semicircular canal epithelium Present Regionally restricted 2c upper center Expression is seen in the hair cells of the anterior crista.
Specimen 2c - lc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: lateral semicircular canal epithelium Present Regionally restricted 2c upper right Expression is seen in the hair cells of the lateral crista.
Specimen 2c - pc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: posterior semicircular canal epithelium Present Regionally restricted 2c lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2d - s: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: saccule epithelium Present Regionally restricted 2d upper left Expression is seen in the hair cells.
Specimen 2d - u: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: utricle epithelium Present Regionally restricted 2d lower left Expression is seen in the hair cells.
Specimen 2d - ac/lc: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: lateral semicircular canal epithelium Present Regionally restricted 2d upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS24: superior semicircular canal epithelium Present Regionally restricted 2d upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2d - pc: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: posterior semicircular canal epithelium Present Regionally restricted 2d lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2e - s: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: saccule epithelium Present Regionally restricted 2e upper left Expression is seen in the hair cells.
Specimen 2e - u: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: utricle epithelium Present Regionally restricted 2e lower left Expression is seen in the hair cells.
Specimen 2e - ac/lc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: lateral semicircular canal epithelium Present Regionally restricted 2e upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS25: superior semicircular canal epithelium Present Regionally restricted 2e upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2e - pc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: posterior semicircular canal epithelium Present Regionally restricted 2e lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2f - s: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: saccule epithelium Present Regionally restricted 2f upper left Expression is seen in the hair cells.
Specimen 2f - u: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: utricle epithelium Present Regionally restricted 2f lower left Expression is seen in the hair cells.
Specimen 2f - lc: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: lateral semicircular canal Present Regionally restricted 2f upper right Expression is seen in the hair cells of the lateral crista.
Specimen 2f - pc: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: posterior semicircular canal Present Regionally restricted 2f lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2g: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS22: cochlea Absent 2g Merge , 2g GFI1 No hair cells were seen in the cochlea at this age.
Specimen 2h: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS23: cochlea Present Regionally restricted 2h GFI1 , 2h Merge Expression is seen in the hair cells of the basal cochlea. (As hair cell differentiation proceeds, expression will expand to the middle and then apical cochlea.)
Specimen 2i: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 2i GFI1 , 2i Merge Expression is seen in the inner and outer hair cells.
Specimen 2j: postnatal day 7; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Double labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266).
Structure Level Pattern Image Note
TS28: cochlea Present Regionally restricted 2j right , 2j center Expression is seen in the inner and outer hair cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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