About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:297267 Tang Q, et al., Gfi1-GCE inducible Cre line for hair cell-specific gene manipulation in mouse inner ear. Genesis. 2019 Sep;57(9):e23304
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6717267
Gene symbol: Gfi1
Gene name: growth factor independent 1 transcription repressor
Antibody: anti-Gfi1 (N-20; sc-8558)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1c: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 1c merge , 1c GFI1 Expression is in the nuclei of the inner and outer hair cells.
Specimen 1e: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1tm2.1(GFP/cre/ERT2)Gan (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 1e merge , 1e GFI1 Expression is in the nuclei of the inner and outer hair cells.
Specimen 2a - u/s: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS21: saccule epithelium Present Regionally restricted 2a left Expression is seen in the hair cells.
TS21: utricle epithelium Present Regionally restricted 2a left Expression is seen in the hair cells.
Specimen 2a - ac: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS21: superior semicircular canal epithelium Present Regionally restricted 2a upper right Expression is seen in the hair cells of the anterior crista.
Specimen 2a - pc: embryonic day 13.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS21: posterior semicircular canal epithelium Present Regionally restricted 2a lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2b - u/s: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS22: saccule epithelium Present Regionally restricted 2b left Expression is seen in the hair cells.
TS22: utricle epithelium Present Regionally restricted 2b left Expression is seen in the hair cells.
Specimen 2b - ac/lc: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 2b upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS22: superior semicircular canal epithelium Present Regionally restricted 2b upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2b - pc: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS22: posterior semicircular canal epithelium Present Regionally restricted 2b lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2c - s: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: saccule epithelium Present Regionally restricted 2c upper left Expression is seen in the hair cells.
Specimen 2c - u: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: utricle epithelium Present Regionally restricted 2c lower left Expression is seen in the hair cells.
Specimen 2c - ac: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: superior semicircular canal epithelium Present Regionally restricted 2c upper center Expression is seen in the hair cells of the anterior crista.
Specimen 2c - lc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: lateral semicircular canal epithelium Present Regionally restricted 2c upper right Expression is seen in the hair cells of the lateral crista.
Specimen 2c - pc: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS23: posterior semicircular canal epithelium Present Regionally restricted 2c lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2d - s: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: saccule epithelium Present Regionally restricted 2d upper left Expression is seen in the hair cells.
Specimen 2d - u: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: utricle epithelium Present Regionally restricted 2d lower left Expression is seen in the hair cells.
Specimen 2d - ac/lc: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: lateral semicircular canal epithelium Present Regionally restricted 2d upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS24: superior semicircular canal epithelium Present Regionally restricted 2d upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2d - pc: embryonic day 16.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS24: posterior semicircular canal epithelium Present Regionally restricted 2d lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2e - s: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: saccule epithelium Present Regionally restricted 2e upper left Expression is seen in the hair cells.
Specimen 2e - u: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: utricle epithelium Present Regionally restricted 2e lower left Expression is seen in the hair cells.
Specimen 2e - ac/lc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: lateral semicircular canal epithelium Present Regionally restricted 2e upper right Expression is seen in the hair cells of the anterior and lateral crista.
TS25: superior semicircular canal epithelium Present Regionally restricted 2e upper right Expression is seen in the hair cells of the anterior and lateral crista.
Specimen 2e - pc: embryonic day 17.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS25: posterior semicircular canal epithelium Present Regionally restricted 2e lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2f - s: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: saccule epithelium Present Regionally restricted 2f upper left Expression is seen in the hair cells.
Specimen 2f - u: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: utricle epithelium Present Regionally restricted 2f lower left Expression is seen in the hair cells.
Specimen 2f - lc: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: lateral semicircular canal Present Regionally restricted 2f upper right Expression is seen in the hair cells of the lateral crista.
Specimen 2f - pc: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Triple labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); blue - DAPI.
Structure Level Pattern Image Note
TS27: posterior semicircular canal Present Regionally restricted 2f lower right Expression is seen in the hair cells of the posterior crista.
Specimen 2g: embryonic day 14.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS22: cochlea Absent 2g Merge , 2g GFI1 No hair cells were seen in the cochlea at this age.
Specimen 2h: embryonic day 15.5; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS23: cochlea Present Regionally restricted 2h GFI1 , 2h Merge Expression is seen in the hair cells of the basal cochlea. (As hair cell differentiation proceeds, expression will expand to the middle and then apical cochlea.)
Specimen 2i: postnatal day 0; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Multi-labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266); gray - Myo6 protein (assay MGI:6717268); blue - DAPI.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 2i GFI1 , 2i Merge Expression is seen in the inner and outer hair cells.
Specimen 2j: postnatal day 7; Gfi1tm2.1(GFP/cre/ERT2)Gan/Gfi1+ (more )
Note: Double labeled: red - Gfi1 protein; green - Gfi1 reporter (assay MGI:6717266).
Structure Level Pattern Image Note
TS28: cochlea Present Regionally restricted 2j right , 2j center Expression is seen in the inner and outer hair cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory