H2ax Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6715676
Gene symbol:	H2ax
Gene name:	H2A.X variant histone

Antibody:	anti-phosphoH2AX (#05-636; JBW301)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

4c Meioc+/+ gamma-H2AX ©

Specimen 4c +/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		4c Meioc+/+ gamma-H2AX

4c Meioc-/- gamma-H2AX ©

Specimen 4c -/-: embryonic day 15.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Weak		4c Meioc-/- gamma-H2AX	The meiotic marker was poorly detected, although germ cell density appeared unaltered.

4e Meioc+/+ Vasa/gamma-H2AX ©

Specimen 4e +/+ VASA/g-H2AX: embryonic day 18.5 (more

)
Note: Double labeled: brown - H2ax; magenta - Ddx4 (assay MGI:6695761).

Structure	Level	Pattern	Image	Note
TS26: meiotic germ cell of ovary	Weak		4e Meioc+/+ Vasa/gamma-H2AX	Expression of H2ax was progressively lost during later fetal life in wild type.

4e Meioc-/- Vasa/gamma-H2AX ©

Specimen 4e -/- VASA/g-H2AX: embryonic day 18.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: brown - H2ax; magenta - Ddx4 (assay MGI:6695761).

Structure	Level	Pattern	Image	Note
TS26: meiotic germ cell of ovary	Present		4e Meioc-/- Vasa/gamma-H2AX	An abundance of staining for H2ax was detected in the mutant.

5a 18.5dpc Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 18.5-dpc +/+: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Absent		5a 18.5dpc Meioc+/+ gamma-H2AX

5a 18.5dpc Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 18.5-dpc -/-: embryonic day 18.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Absent		5a 18.5dpc Meioc-/- gamma-H2AX

5a 6dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 6-dpp +/+: postnatal day 6 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Absent		5a 6dpp Meioc+/+ gamma-H2AX

5a 6dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 6-dpp -/-: postnatal day 6; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Absent		5a 6dpp Meioc-/- gamma-H2AX

5a 8dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 8-dpp +/+: postnatal day 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 8dpp Meioc+/+ gamma-H2AX	Meiosis initiated at 8 dpp.

5a 8dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 8-dpp -/-: postnatal day 8; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Weak		5a 8dpp Meioc-/- gamma-H2AX	At 8 dpp H2ax appeared to be globally weaker in the mutant than in wild type testes.

5a 10dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 10-dpp +/+: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 10dpp Meioc+/+ gamma-H2AX

5a 10dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 10-dpp -/-: postnatal day 10; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 10dpp Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

5a 16dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 16-dpp +/+: postnatal day 16 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 16dpp Meioc+/+ gamma-H2AX

5a 16dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 16-dpp -/-: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 16dpp Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

5a 20dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 20-dpp +/+: postnatal day 20 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 20dpp Meioc+/+ gamma-H2AX

Specimen 5a g-H2AX 20-dpp -/-: postnatal day 20; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 20dpp Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

Specimen 5a g-H2AX Adult +/+: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a Adult Meioc+/+ gamma-H2AX

Specimen 5a g-H2AX Adult -/-: postnatal adult; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a Adult Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

7a right ©

Specimen 7a: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7a right

S4e +/+ ©

Specimen S4e +/+: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4e +/+

S4e -/- ©

Specimen S4e -/-: postnatal adult; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4e -/-	All spermatocytes were positive for H2ax in the mutant testis.

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