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Gene Expression Data
Assay Details
Assay
Reference: J:241850 Naiman N, et al., Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development. Dev Cell. 2017 May 22;41(4):349-365.e3
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6711765
Gene symbol: Six2
Gene name: sine oculis-related homeobox 2
Antibody: anti-Six2 (11562-1-AP)
Detection system: Secondary antibody coupled to DyLight
Results
Specimen 1B/D: embryonic day 10.5 (more )
Note: Triple labeled: green - Six2; red - Pax2 (assay MGI:6711810); blue - Foxd1 (assay MGI:6711779)
Structure Level Pattern Image Note
TS17: metanephric mesenchyme Present 1D , 1B
Specimen 1F/H: embryonic day 11.5 (more )
Note: Triple labeled: green - Six2; red - Pax2 (assay MGI:6711810); blue - Foxd1 (assay MGI:6711779)
Structure Level Pattern Image Note
TS19: cap mesenchyme Present 1F , 1H
Specimen 1J/L: embryonic day 15.5 (more )
Note: Triple labeled: green - Six2; red - Pax2 (assay MGI:6711810); blue - Foxd1 (assay MGI:6711779)
Structure Level Pattern Image Note
TS23: cap mesenchyme Present 1J , 1L
Specimen 2I: embryonic day 18.5; Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Foxd1 (assay MGI:6711779); white - cytokeratin
Structure Level Pattern Image Note
TS26: cap mesenchyme Present 2I
TS26: renal cortical interstitium Absent 2I
Specimen 2J: embryonic day 18.5; Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Foxd1 (assay MGI:6711779); white - cytokeratin
Structure Level Pattern Image Note
TS26: cap mesenchyme Present Single cells 2J Expression is seen in a few stray cells adjacent to the rare ureteric tip.
TS26: renal cortical interstitium Absent 2J
Specimen 4C: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Cre reporter; white - cytokeratin
Structure Level Pattern Image Note
TS26: cap mesenchyme Present 4C
Specimen 4D: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Cre reporter; white - cytokeratin
Structure Level Pattern Image Note
TS26: cap mesenchyme Absent 4D
Specimen 5A/E/G: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Foxd1 (assay MGI:6711779); blue - Cre reporter
Structure Level Pattern Image Note
TS21: cap mesenchyme Present 5G , 5E , 5A
TS21: renal interstitium Absent 5A , 5G , 5E Any expression in interstitial cells is a rare event.
Specimen 5B/F/H: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Foxd1 (assay MGI:6711779); blue - Cre reporter
Structure Level Pattern Image Note
TS21: cap mesenchyme Present Single cells 5H , 5B , 5F Expression is seen in the rare cells that escaped inactivation.
TS21: renal interstitium Present Single cells 5B , 5F , 5H
Specimen S2A'/A'''/A''''': embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Six2 antibody; green - Cre reporter; blue - Pax2 (assay MGI:6711810)
Structure Level Pattern Image Note
TS26: cap mesenchyme Present S2A' , S2A''' , S2A'''''
Specimen S2B'/B'''/B''''': embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Six2 antibody; green - Cre reporter; blue - Pax2 (assay MGI:6711810)
Structure Level Pattern Image Note
TS26: cap mesenchyme Present Single cells S2B' , S2B''''' , S2B''' Expression is seen in the rare cells that escaped inactivation.
Specimen S2C: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; blue - Foxd1 (assay MGI:6711779); red - Cre reporter
Structure Level Pattern Image Note
TS26: cap mesenchyme Present S2C
Specimen S2D: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; blue - Foxd1 (assay MGI:6711779); red - Cre reporter
Structure Level Pattern Image Note
TS26: cap mesenchyme Present Single cells S2D Expression is seen in the rare cells that escaped inactivation.
Specimen S3Q/Q''/Q''': embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2+, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Foxd1 (assay MGI:6711779); blue - Cre reporter
Structure Level Pattern Image Note
TS23: cap mesenchyme Present S3Q , S3Q'' , S3Q'''
TS23: renal cortical interstitium Absent S3Q , S3Q''' , S3Q'' Any expression in interstitial cells is a rare event.
Specimen S3R/R''/R''': embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pax2tm1.1Akio/Pax2tm1.2Akio, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Six2; red - Foxd1 (assay MGI:6711779); blue - Cre reporter
Structure Level Pattern Image Note
TS23: cap mesenchyme Present Single cells S3R , S3R''' , S3R'' Expression is seen in the rare cells that escaped inactivation.
TS23: renal cortical interstitium Present Single cells S3R'' , S3R''' , S3R
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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