Casp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:297493 Matos-Rodrigues GE, et al., Progenitor death drives retinal dysplasia and neuronal degeneration in a mouse model of ATRIP-Seckel syndrome. Dis Model Mech. 2020 Oct 30;13(10):dmm045807
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6506439
Gene symbol:	Casp3
Gene name:	caspase 3

Antibody:	anti-caspase-3 (clone C92-605)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes:	The antibody recognized the cleaved form of the protein.

Results

4C Atrip^Ctrl ©

Specimen 4C Atrip^Ctrl: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: retina	Ambiguous		4C Atrip^Ctrl

Specimen 4C Atrip^alpha-Cre: embryonic day 17.5; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: retina	Present		4C Atrip^alpha-Cre	A substantial increase in apoptosis (~50-fold) was detected in the mutant retina.

Specimen 4D E12.5 Atrip^Ctrl: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: retina	Ambiguous

Specimen 4D E12.5 Atrip^alpha-Cre: embryonic day 12.5; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: retina	Ambiguous			No change in apoptosis was detected in the mutant retina.

Specimen 4D P2 Atrip^Ctrl: postnatal day 2 (more

)

Structure	Level	Pattern	Image	Note
TS27: retina	Present

Specimen 4D P2 Atrip^alpha-Cre: postnatal day 2; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: retina	Present			A modest increase in apoptosis was detected in the mutant retina.

Specimen 4D P4 Atrip^Ctrl: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present

Specimen 4D P4 Atrip^alpha-Cre: postnatal day 4; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: retina	Present			A modest increase in apoptosis was detected in the mutant retina.

Specimen S3B Atrip^Ctrl: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: retina	Ambiguous		S3B Atrip^Ctrl

Specimen S3B Atrip^alpha-Cre: embryonic day 17.5; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: retina	Present	Regionally restricted	S3B Atrip^alpha-Cre	Apoptosis was in the retinal periphery.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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