Gene Expression Data
Assay Details
Assay
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Reference:
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J:32058
Kilpatrick TJ, et al., Expression of the Tyro4/Mek4/Cek4 gene specifically marks a subset of embryonic motor neurons and their muscle targets. Mol Cell Neurosci. 1996 Jan;7(1):62-74
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6505441
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Gene symbol:
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Epha3
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Gene name:
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Eph receptor A3
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Probe:
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Mek4 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5A:
embryonic day 10.5
(more )
Specimen
5A:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
embryonic day 12.5
(more )
Specimen
5B:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5C:
embryonic day 15.5
(more )
Specimen
5C:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: ventral grey horn |
Present |
Regionally restricted |
5C
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Expression is in motor neurons. Highest expression is in the cervical and thoracic regions.
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Specimen
6C:
embryonic day 12.5
(more )
Specimen
6C:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: basal columns |
Present |
Regionally restricted |
6C
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Expression is in the medial motor cloumns at the level of the brachial spinal cord.
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Specimen
7B:
embryonic day 12.5
(more )
Specimen
7B:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: basal columns |
Present |
Regionally restricted |
7B
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Expression is in a subset of neurons in the medial motor cloumns at the level of the cervical spinal cord.
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Specimen
9B:
embryonic day 12.5
(more )
Specimen
9B:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: skeletal musculature |
Present |
Regionally restricted |
9B
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Expression is in a subset of the axial musculature. Expression is seen in the rhomboideus capitis, deltoids, and trapezius and is absent in the splenius capitis, cervicus capitis, rectus cervicis, and subcapsularis.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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