Antibody:	anti-AQP5 (AQP-005)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Absent		1A
TS22: submandibular gland primordium epithelium	Absent		1A
TS22: submandibular gland primordium mesenchyme	Absent		1A

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Absent		1B
TS22: submandibular gland primordium epithelium	Absent		1B
TS22: submandibular gland primordium mesenchyme	Absent		1B

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium	Present	Scattered	1C	There are scattered cells in the terminal bud and there is no expression in the presumptive duct.
TS23: blood vessel	Absent		1C
TS23: submandibular gland primordium mesenchyme	Absent		1C

Structure	Level	Pattern	Image	Note
TS24: submandibular gland epithelium	Present	Regionally restricted	1D	Expression is in all pro-acinar cells.
TS24: submandibular gland	Present	Regionally restricted	1D	Expression is in cells proximal to the pro-acini in the intralobular duct.
TS24: blood vessel	Absent		1D
TS24: submandibular gland mesenchyme	Absent		1D

Structure	Level	Pattern	Image	Note
TS25: submandibular gland epithelium	Present	Regionally restricted	1E	Expression is in pro-acinar cells.
TS25: submandibular gland	Present	Regionally restricted	1E	Expression is in cells proximal to the pro-acini in the intralobular duct and is absent in the interlobular duct.
TS25: blood vessel	Absent		1E
TS25: submandibular gland mesenchyme	Absent		1E

Structure	Level	Pattern	Image	Note
TS25: submandibular gland epithelium	Present	Regionally restricted	1F	Expression is in pro-acinar cells.
TS25: submandibular gland	Present	Regionally restricted	1F	Expression is in cells proximal to the pro-acini in the intralobular duct and is absent in the interlobular duct.
TS25: blood vessel	Absent		1F
TS25: submandibular gland mesenchyme	Absent		1F

Structure	Level	Pattern	Image	Note
TS27: salivary gland epithelium	Present	Regionally restricted	2A	Expression is apical and lateral in pro-acinar cells with weaker staining in the basal membrane and cytoplasm.
TS27: submandibular duct	Present	Regionally restricted	2A	Expression is in the intercalated ducts and is absent in the intralobular duct, interlobular duct, and the main excretory duct.

Structure	Level	Pattern	Image	Note
TS28: salivary gland epithelium	Present	Regionally restricted	2B	Expression is in pro-acinar cells.
TS28: submandibular duct	Present	Regionally restricted	2B	Expression is in the intercalated ducts and is absent in the intralobular duct, interlobular duct, and the main excretory duct.

Structure	Level	Pattern	Image	Note
TS28: salivary gland epithelium	Present		2C	Expression is in acini apical and lateral membrane with weaker expression in the basal membrane and in the cytoplasm.
TS28: submandibular duct	Present	Regionally restricted	2C	Expression is in the proximal part of the intercalated duct and is absent in the granulated convoluted tubule.

Structure	Level	Pattern	Image	Note
TS28: salivary gland epithelium	Present	Regionally restricted	2D	Expression is in the luminal, lateral, and basal membrane of the acinar cells.
TS28: submandibular duct	Present	Regionally restricted	2D	Expression is in the proximal part of the intercalated duct and is absent in the granulated convoluted tubule and absent in the transition from the granulated convoluted tubule to striated duct.

Structure	Level	Pattern	Image	Note
TS28: salivary gland epithelium	Present	Regionally restricted	2E	Expression is in the luminal, lateral, and basal membrane of the acinar cells.
TS28: submandibular duct	Present	Regionally restricted	2E	Expression is in the entire intercalated ducts and is absent in the granulated convoluted tubule.

Structure	Level	Pattern	Image	Note
TS27: salivary gland epithelium	Present	Regionally restricted		Expression is apical and lateral in pro-acinar cells with weaker staining in the basal membrane and cytoplasm.
TS27: submandibular duct	Present	Regionally restricted		Expression is in the intercalated ducts and is absent in the intralobular duct, interlobular duct, and the main excretory duct.

Structure	Level	Pattern	Image	Note
TS28: salivary gland epithelium	Present	Regionally restricted		Expression is in acini apical and lateral membrane with weaker expression in the basal membrane and in the cytoplasm.
TS28: submandibular duct	Present	Regionally restricted		Expression is in the proximal part of the intercalated duct and is absent in the granulated convoluted tubule.