Antibody:	anti-AR
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit 2	Present	Widespread	2A	Expression is present in ~90% of the cells, with most of the cells showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels and type of expression are comparable between males and females.
TS20: hindlimb digit 4	Present	Widespread	2A , 2C	Expression is present in ~90% of the cells, with most of the cells showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels and type of expression are comparable between males and females.

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit 2	Present	Widespread	2B	Expression is present in ~90% of the cells, with most of the cells showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels and type of expression are comparable between males and females.
TS20: hindlimb digit 4	Present	Widespread	2B	Expression is present in ~90% of the cells, with most of the cells showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels and type of expression are comparable between males and females.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit 2	Present	Widespread	2E	Expression is present in most of the cells, with most showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels in both digits in males are higher than those seen in females.
TS22: hindlimb digit 4	Present	Widespread	2G , 2E	Expression is present in most of the cells, with most showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels in both digits in males are higher than those seen in females.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit 2	Present	Widespread	2F	Expression is present in many of the cells, with most showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels in both digits in females are lower than those seen in males.
TS22: hindlimb digit 4	Present	Widespread	2F	Expression is present in many of the cells, with most showing activated (nuclear) localization. The 4th digit has higher levels of activation than the second digit. Expression levels in both digits in females are lower than those seen in males.

Structure	Level	Pattern	Image	Note
TS24: hindlimb digit 2	Absent		S1A left	No expression is seen in the hypertrophic zone of the proximal phalange.

Structure	Level	Pattern	Image	Note
TS24: hindlimb digit 4	Absent		S1C , S1A right	No expression is seen in the hypertrophic zone of the proximal phalange.

Structure	Level	Pattern	Image	Note
TS24: hindlimb digit 2	Absent		S1B left	No expression is seen in the hypertrophic zone of the proximal phalange.

Structure	Level	Pattern	Image	Note
TS24: hindlimb digit 4	Absent		S1B right	No expression is seen in the hypertrophic zone of the proximal phalange.