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Gene Expression Data
Assay Details
Assay
Reference: J:110696 Gonzalez MA, et al., Geminin is essential to prevent endoreduplication and to form pluripotent cells during mammalian development. Genes Dev. 2006 Jul 15;20(14):1880-4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6470686
Gene symbol: Gmnn
Gene name: geminin
Antibody: anti-Geminin
Results
Specimen 2B 8-cell WT: embryonic day 2.5 (more )
Note: Triple labeled: green - Gmnn; blue - Krt8 (Troma-1; assay MGI:6470685); red - DAPI.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2B 8-cell WT Geminin , 2B 8-cell WT Merge
Specimen 2B 8-cell Mutant: embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Triple labeled: green - Gmnn; blue - Krt8 (Troma-1; assay MGI:6470685); red - DAPI.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2B 8-cell Mutant Geminin , 2B 8-cell Mutant Merge
Specimen 2B 32-cell WT: embryonic day 3.5 (more )
Note: Triple labeled: green - Gmnn; blue - Krt8 (Troma-1; assay MGI:6470685); red - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present 2B 32-cell WT Geminin , 2B 32-cell WT Merge
Specimen 3 8-cell Mutant: embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Double labeled: green - Geminin (Gmnn); red - DAPI.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 3_3d reconstruction , 3_optical section Authors report absence of nuclear expression.
Specimen 4A WT: embryonic day 3.5 (more )
Note: Double labeled: green - Geminin (Gmnn); red - Oct4 (Pou5f1 assay MGI:6470688).
Structure Level Pattern Image Note
TS4: blastocyst Present 4A WT Geminin , 4A WT Merge
Specimen 4A Mutant: embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Double labeled: green - Geminin (Gmnn); red - Oct4 (Pou5f1 assay MGI:6470688).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 4A Mutant Merge , 4A Mutant Geminin
Specimen 4B WT: embryonic day 3.5 (more )
Note: Double labeled: green - Geminin (Gmnn); red - Cdx2 (assay MGI:6470689).
Structure Level Pattern Image Note
TS4: blastocyst Present 4B WT Geminin , 4B WT Merge
Specimen 4B Mutant: embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Double labeled: green - Geminin (Gmnn); red - Cdx2 (assay MGI:6470689).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 4B Mutant Geminin , 4B Mutant Merge
Specimen S4 2-cell: embryonic day 1.5 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted S4_2-cell Expression in innerphase nuclei of all blastomeres.
Specimen S4 4-cell (left): embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted S4_4-cell Geminin (left) Expression in innerphase nuclei of all blastomeres.
Specimen S4 4-cell (middle): embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted S4_4-cell Geminin (middle) Expression in innerphase nuclei of all blastomeres.
Specimen S4 4-cell (right): embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted S4_4-cell Geminin (right) Expression in innerphase nuclei of all blastomeres.
Specimen S4 8-cell (left): embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted S4_8-cell Geminin (left) Expression in innerphase nuclei of all blastomeres.
Specimen S4 8-cell (left middle): embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted S4_8-cell Geminin (left middle) Expression in innerphase nuclei of all blastomeres.
Specimen S4 8-cell (middle right): embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted S4_8-cell Geminin (middle right) Expression in innerphase nuclei of all blastomeres.
Specimen S4 8-cell (right): embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted S4_8-cell Geminin (right)
Specimen S4 16-cell: embryonic day 3.0 (more )
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted S4_16-cell Geminin Expression in innerphase nuclei of all blastomeres.
Specimen S4 >32-cell: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted S4_>32-cell Geminin Expression in innerphase nuclei of all blastomeres.
Specimen S5 WT: embryonic day 3.5 (more )
Note: Double labeled: red - Geminin (Gmnn); green - p57Kip2 (Cdkn1c assay MGI:6470690).
Structure Level Pattern Image Note
TS4: blastocyst Present S5_WT p57Kip2 Geminin
Specimen S5 Mutant: embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Double labeled: red - Geminin (Gmnn); green - p57Kip2 (Cdkn1c assay MGI:6470690).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent S5_Mutant p57Kip2 Geminin
Specimen S5 Optical section (left): embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Double labeled: red - Geminin (Gmnn); green - p57Kip2 (Cdkn1c assay MGI:6470690).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent S5_Optical section (left)
Specimen S5 Optical section (middle): embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Double labeled: red - Geminin (Gmnn); green - p57Kip2 (Cdkn1c assay MGI:6470690).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent S5_Optical section (middle)
Specimen S5 Optical section (right): embryonic day 3.5; GmnnGt(XT0615)Wtsi/GmnnGt(XT0615)Wtsi (more )
Note: Double labeled: red - Geminin (Gmnn); green - p57Kip2 (Cdkn1c assay MGI:6470690).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent S5_Optical section (right)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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