Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:110696 Gonzalez MA, et al., Geminin is essential to prevent endoreduplication and to form pluripotent cells during mammalian development. Genes Dev. 2006 Jul 15;20(14):1880-4
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6470688
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:

anti-Oct4

Results

Specimen 4A WT: embryonic day 3.5 (more

)
Note: Double labeled: red - Oct4 (Pou5f1); green - Geminin (Gmnn assay MGI:6470686).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		4A WT Oct4 , 4A WT Merge	Expression in all blastomeres of cleavage stage embryos.

Specimen 4A Mutant: embryonic day 3.5; Gmnn^{Gt(XT0615)Wtsi}/Gmnn^{Gt(XT0615)Wtsi} (more

)
Note: Double labeled: red - Oct4 (Pou5f1); green - Geminin (Gmnn assay MGI:6470686).

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Absent		4A Mutant Merge , 4A Mutant Oct4

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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