Nkx2-2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:262122 Gigante ED, et al., Hypomorphic Smo mutant with inefficient ciliary enrichment disrupts the highest level of vertebrate Hedgehog response. Dev Biol. 2018 May 15;437(2):152-162
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6469354
Gene symbol:	Nkx2-2
Gene name:	NK2 homeobox 2

Antibody:	74.5A5
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

3E ©

Specimen 3E: embryonic day 10.5; Smo^cbb/Smo⁺ (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6469353); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3E

3F ©

Specimen 3F: embryonic day 10.5; Smo^cbb/Smo^cbb (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6469353); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present	Regionally restricted	3F	Some cells co-expressing Nkx2.2.

3G ©

Specimen 3G: embryonic day 10.5; Smo^cbb/Smo⁺ (more

)
Note: Triple labeled: green - Nkx2-2; red - Olig2 (assay MGI:6469355); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3G

3H ©

Specimen 3H: embryonic day 10.5; Smo^cbb/Smo^cbb (more

)
Note: Triple labeled: green - Nkx2-2; red - Olig2 (assay MGI:6469355); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present	Regionally restricted	3H	Expression in cells at midline.

3I ©

Specimen 3I: embryonic day 11.5; Smo^cbb/Smo⁺ (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6469353); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	3I

3J ©

Specimen 3J: embryonic day 11.5; Smo^cbb/Smo^cbb (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:6469353); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	3J	No co-expression with Foxa2.

3K ©

Specimen 3K: embryonic day 11.5; Smo^cbb/Smo⁺ (more

)
Note: Triple labeled: green - Nkx2-2; red - Olig2 (assay MGI:6469355); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	3K	Expression domain adjacent to Olig2 domain.

3L ©

Specimen 3L: embryonic day 11.5; Smo^cbb/Smo^cbb (more

)
Note: Triple labeled: green - Nkx2-2; red - Olig2 (assay MGI:6469355); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	3L	Expression domain adjacent to Olig2 domain.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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