Gene Expression Data
Assay Details
Assay
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Reference:
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J:49214
Sheward WJ, et al., Expression of PACAP, and PACAP type 1 (PAC1) receptor mRNA during development of the mouse embryo. Brain Res Dev Brain Res. 1998 Aug 8;109(2):245-53
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6468228
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Gene symbol:
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Adcyap1
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Gene name:
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adenylate cyclase activating polypeptide 1
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Probe:
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PACAP probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3A:
embryonic day 11.5
(more )
Specimen
3A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B:
embryonic day 11.5
(more )
Specimen
3B:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
3B
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Expression is seen in the ventral (hypothalamic) portion of the diencephalon.
|
TS19: hindbrain |
Present |
Regionally restricted |
3B
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Expression is seen in a discontinuous pattern in the floor of the rhombencephalon.
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Specimen
3C:
embryonic day 11.5
(more )
Specimen
3C:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
3C
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Expression is seen in the ventral (hypothalamic) portion of the diencephalon.
|
TS19: hindbrain |
Present |
Regionally restricted |
3C
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Expression is seen in a discontinuous pattern in the floor of the rhombencephalon.
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Specimen
3D:
embryonic day 11.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
3D
|
Expression is seen in the ventral (hypothalamic) portion of the diencephalon.
|
TS19: hindbrain |
Present |
Regionally restricted |
3D
|
Expression is seen in a discontinuous pattern in the floor of the rhombencephalon.
|
Specimen
3E:
embryonic day 11.5
(more )
Specimen
3E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Specimen
3F:
embryonic day 11.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Strong |
Regionally restricted |
3F
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Expression is seen in the ventral (hypothalamic) portion of the diencephalon.
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TS19: metencephalon |
Present |
Regionally restricted |
3F
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Expression is seen in the nuclei of the pons.
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TS19: hindbrain |
Present |
Regionally restricted |
3F
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Expression is seen in the nuclei of the medulla.
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TS19: trigeminal ganglion |
Present |
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3F
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4B:
embryonic day 11.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
4B
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Expression is seen in two columns of cells in the ventromedial portion of the neural tube (consistent with the position of developing autonomic motoneurons) and in a large group of cells in the dorsolateral portion of the neural tube.
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