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Gene Expression Data
Assay Details
Assay
Reference: J:275903 Shiura H, et al., Xist/Tsix expression dynamics during mouse peri-implantation development revealed by whole-mount 3D RNA-FISH. Sci Rep. 2019 Mar 6;9(1):3637
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6459718
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (N-19; sc-8628)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 1b: embryonic day 3.5 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1b marker , 1b merge
Specimen 1c: embryonic day 3.75 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1c merge , 1c marker
Specimen 1e: embryonic day 5.0 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS7: epiblast Present 1e merge , 1e marker
Specimen 1f: embryonic day 5.25 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS8: epiblast Present 1f marker , 1f merge
Specimen 1d,h: embryonic day 4.75 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS7: epiblast Present 1d marker , 1h , 1d merge
Specimen 1g,i: embryonic day 5.5 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS8: epiblast Present 1i , 1g merge , 1g marker
Specimen S1a E3.5: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present S1a POU5F1 E3.5
Specimen S1a E4.75: embryonic day 4.75 (more )
Structure Level Pattern Image Note
TS7: epiblast Present S1a POU5F1 E4.75
Specimen S1a E5.0: embryonic day 5.0 (more )
Structure Level Pattern Image Note
TS7: epiblast Present S1a POU5F1 E5.0
Specimen S1a E5.25: embryonic day 5.25 (more )
Structure Level Pattern Image Note
TS8: epiblast Present S1a POU5F1 E5.25
Specimen S1a E5.5: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS8: epiblast Present S1a POU5F1 E5.5
Specimen S3b: embryonic day 5.25 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS8: epiblast Present S3b Merge , S3b marker
Specimen S3c: embryonic day 5.5 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS8: epiblast Present S3c marker , S3c Merge
Specimen S4a: embryonic day 5.5 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS8: epiblast Present S4a marker , S4a Merge , S4a left
Specimen S4b: embryonic day 5.5 (more )
Note: Triple labeled: blue- Pou5f1; green - Xist (assay MGI:6459735); magenta - Tsix (assay MGI:6459736).
Structure Level Pattern Image Note
TS8: epiblast Present S4b Merge , S4b marker , S4b left
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory