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Gene Expression Data
Assay Details
Assay
Reference: J:275903 Shiura H, et al., Xist/Tsix expression dynamics during mouse peri-implantation development revealed by whole-mount 3D RNA-FISH. Sci Rep. 2019 Mar 6;9(1):3637
Assay type: RNA in situ
MGI Accession ID: MGI:6459736
Gene symbol: Tsix
Gene name: X (inactive)-specific transcript, opposite strand
Probe: Tsix probe
Probe preparation: Antisense labelled with Cy3 RNA
Visualized with: Fluorescence
Assay notes: Single molecule fluorescence in situ hybridization (FISH) analysis. Probe was labeled with Green-dUTP.
Results
Specimen 1b: embryonic day 3.5 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1b Tsix , 1b merge More than 80% of inner cell mass cells had one Xist cloud and one pinpoint Tsix signal per nucleus. Shown is a single cell with one Xist cloud and a single Tsix signal.
Specimen 1c: embryonic day 3.75 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1c Tsix , 1c merge Nuclear cloud signals began to disappear and cells showing biallelic expression of Tsix began to appear. Shown is a single cell with no Xist and a single Tsix signal.
Specimen 1e: embryonic day 5.0 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS7: epiblast Present 1e merge , 1e Tsix Shown is a single cell with one Xist pinpoint signal and two Tsix signals. 26% of the epiblast cells showed weak Xist expression as a pinpoint signal.
Specimen 1f: embryonic day 5.25 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS8: epiblast Present 1f merge , 1f Tsix More than half of the cells (55%) had Xist signals with most of the cells showing dispersed Xist signals. Cells with Xist clouds began to appear. Shown is a single cell with one Xist dispersed signal and a single Tsix spot.
Specimen 1d,h: embryonic day 4.75 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS7: epiblast Present 1d Tsix , 1d merge , 1h Encircled is a single cell with two Xist clouds and a single Tsix signal.
Specimen 1g,i: embryonic day 5.5 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS8: epiblast Present 1g merge , 1g Tsix , 1i Encircled is a cell with two Xist clouds and a single Tsix signal.
Specimen 2c: embryonic day 5.0 (more )
Note: Triple labeled: magenta - Tsix; blue - Gata6 (assay MGI:6459723); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS7: epiblast Present 2c
Specimen 2d: embryonic day 4.25 (more )
Note: Triple labeled: magenta - Tsix; blue - Cdx2 (assay MGI:6459726); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS6: epiblast Present 2d Most of the Trophectoderm/Extraembryonic Ectoderm cells (70-95%) had one Xist cloud and a single Tsix spot.
Specimen 2e: embryonic day 4.5 (more )
Note: Triple labeled: magenta - Tsix; blue - Cdx2 (assay MGI:6459726); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS6: epiblast Present 2f , 2e , 2g Most of the Trophectoderm/Extraembryonic Ectoderm cells (70-95%) had one Xist cloud and a single Tsix spot.
Specimen S3b: embryonic day 5.25 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS8: epiblast Present S3b Merge , S3b Tsix
Specimen S3c: embryonic day 5.5 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS8: epiblast Present S3c Tsix , S3c Merge
Specimen S4a: embryonic day 5.5 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS8: epiblast Present S4a left , S4a Tsix , S4a Merge
Specimen S4b: embryonic day 5.5 (more )
Note: Triple labeled: magenta - Tsix; blue - Pou5f1 (assay MGI:6459718); green - Xist (assay MGI:6459735).
Structure Level Pattern Image Note
TS8: epiblast Present S4b Tsix , S4b left , S4b Merge
Specimen Matrix Figure 1a E4.0: embryonic day 4.0 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present
Specimen Matrix Figure 1a E4.25: embryonic day 4.25 (more )
Structure Level Pattern Image Note
TS6: epiblast Present
Specimen Matrix Figure 1a E4.5: embryonic day 4.5 (more )
Structure Level Pattern Image Note
TS6: epiblast Present 58% of the cells had a single Tsix signal, and 11% and 31% of cells exhibited no or two Tsix signals, respectively.
Specimen Matrix Figure 1a E6.5: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: embryo Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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