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Gene Expression Data
Assay Details
Assay
Reference: J:136688 Buttler K, et al., Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics. BMC Dev Biol. 2008;8:43
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6454345
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: Anti-Prox1 (102-PA32)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1C: embryonic day 13.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6454343).
Structure Level Pattern Image Note
TS22: jugular lymph sac Present 1C Lymphatic endothelial cells in the jugular lymph sacs co-express Prox1 and Lyve1.

Specimen 1D: embryonic day 12.5 (more )
Note: Double labeled: red - Prox1; green - LA102, an epitope on lymphatic endothelial cells.
Structure Level Pattern Image Note
TS20: lymph sac Present Regionally restricted 1D Lymphatic endothelial cells in the jugular lymph sacs were double positive for Prox1 and LA102.

Specimen 2A: embryonic day 10.0 (more )
Note: Double labeled: red - Prox1; green - Pecam1 (assay MGI:6454347).
Structure Level Pattern Image Note
TS17: dermatome Present 2A , 2B A small number of Prox1+/Pecam1+ mesenchymal cells were in the dermatomes.

Specimen 2C: embryonic day 13.5 (more )
Note: Double labeled: red - Prox1; green - LA102, an epitope on lymphatic endothelial cells.
Structure Level Pattern Image Note
TS21: embryo mesenchyme Present Regionally restricted 2C Double positive Prox1+/LA102+ cells were observed in the scattered cells in the mesenchyme.

Specimen 2E: embryonic day 12.5 (more )
Note: Double labeled: red - Prox1; green - Itgam (assay MGI:6454348).
Structure Level Pattern Image Note
TS20: embryo mesenchyme Present 2E A small number of Prox1+/Itgam+ cells were detected in the mesenchyme.

Specimen 4A: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Adgre1 (assay MGI:6454672).
Structure Level Pattern Image Note
TS19: lymph sac Present 4A Cells in the lymph sac expressed Adgre1 only or co-expressed Adgre1 and Prox1.

Specimen 4B: embryonic day 13.5 (more )
Note: Triple labeled: red - Prox1; green - Adgre1 (assay MGI:6454672); blue - Lyve1 (assay MGI:6454672).
Structure Level Pattern Image Note
TS22: jugular lymph sac Present 4D , 4B , 4C Cells were found in the developing lymph sacs that were positive for all three markers.

Specimen 5A: embryonic day 13.5 (more )
Note: Triple labeled: red - Prox1; green - Adgre1 (assay MGI:6454672); blue - Lyve1 (assay MGI:6454672).
Structure Level Pattern Image Note
TS22: lymphatic vessel Present 5A In developing lymph vessels, a small number of cells were identified that were positive for all three markers. In very rare cases, various marker combinations were observed in adjacent cells of lymph vessels. e.g. 3 adjacent cells: one positive for Adgre1 only, one positive for Adgre1 and Lyve1, and a third positive for all three markers.

Specimen 5B: embryonic day 13.5 (more )
Note: Triple labeled: red - Prox1; green - Adgre1 (assay MGI:6454672); blue - Lyve1 (assay MGI:6454672).
Structure Level Pattern Image Note
TS22: lymphatic vessel Present 5B In developing lymph vessels, a small number of cells were identified that were positive for all three markers.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory