Antibody:	Anti F4/80 (BM8)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS21: dermatome	Present	Scattered	3	A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
TS21: mediastinum	Present	Scattered	3	A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
TS21: meninges	Present	Scattered	3	A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present		4A	Cells in the lymph sac expressed Adgre1 only or co-expressed Adgre1 and Prox1.

Structure	Level	Pattern	Image	Note
TS22: jugular lymph sac	Present		4F , 4B , 4C	Cells were found in the developing lymph sacs that were positive for all three markers.

Structure	Level	Pattern	Image	Note
TS22: lymphatic vessel	Present		5A	In developing lymph vessels, a small number of cells were identified that were positive for all three markers. In very rare cases, various marker combinations were observed in adjacent cells of lymph vessels. e.g. 3 adjacent cells: one positive for Adgre1 only, one positive for Adgre1 and Lyve1, and a third positive for all three markers.

Structure	Level	Pattern	Image	Note
TS22: lymphatic vessel	Present		5B	In developing lymph vessels, a small number of cells were identified that were positive for all three markers.