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Gene Expression Data
Assay Details
Assay
Reference: J:231259 Krill KT, et al., Dicer deficiency reveals microRNAs predicted to control gene expression in the developing adrenal cortex. Mol Endocrinol. 2013 May;27(5):754-68
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6451184
Gene symbol: Nr5a1
Gene name: nuclear receptor subfamily 5, group A, member 1
Antibody: anti-SF1
Detection system: Secondary antibody coupled to DyLight 488
Results
Specimen 1C E14.5 Cre- WT: embryonic day 14.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: green - Nr5a1; red - Th (assay MGI:6451175). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS22: adrenal cortex Present 1C upper left
Specimen 1C E14.5 Cre+ KO: embryonic day 14.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nr5a1; red - Th (assay MGI:6451175).
Structure Level Pattern Image Note
TS22: adrenal cortex Present 1C lower left The adrenal cortex appeared to be intact.
Specimen 1C E16.5 Cre- WT: embryonic day 16.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: green - Nr5a1; red - Th (assay MGI:6451175). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1C upper middle
Specimen 1C E16.5 Cre+ KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nr5a1; red - Th (assay MGI:6451175).
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1C lower middle Positive cortex was dramatically thinner compared with control mice.
Specimen 1C E18.5 Cre- WT: embryonic day 18.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: green - Nr5a1; red - Th (assay MGI:6451175). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS26: adrenal cortex Present 1C upper right
Specimen 1C E18.5 Cre+ KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nr5a1; red - Th (assay MGI:6451175).
Structure Level Pattern Image Note
TS26: adrenal cortex Present 1C lower right Very few residual positive-adrenocortical cells.
Specimen 2B 14.5 Cre- WT: embryonic day 14.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: green - Nr5a1; red - Pcna (assay MGI:6451189). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS22: adrenal cortex Present 2B upper left
Specimen 2B 14.5 Cre+ KO: embryonic day 14.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nr5a1; red - Pcna (assay MGI:6451189).
Structure Level Pattern Image Note
TS22: adrenal cortex Present 2B lower left Same as in the control.
Specimen 2B 16.5 Cre- WT: embryonic day 16.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: green - Nr5a1; red - Pcna (assay MGI:6451189). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 2B upper middle
Specimen 2B 16.5 Cre+ KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nr5a1; red - Pcna (assay MGI:6451189).
Structure Level Pattern Image Note
TS24: adrenal cortex Present 2B lower middle Positive cortex was dramatically thinner compared with control mice.
Specimen 2B 18.5 Cre- WT: embryonic day 18.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: green - Nr5a1; red - Pcna (assay MGI:6451189). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS26: adrenal cortex Present 2B upper right
Specimen 2B 18.5 Cre+ KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nr5a1; red - Pcna (assay MGI:6451189).
Structure Level Pattern Image Note
TS26: adrenal cortex Present 2B lower right Very few residual positive-adrenocortical cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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