Gene Expression Data
Assay Details
Assay
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Reference:
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J:223183
Yang T, et al., Analysis of PRICKLE1 in human cleft palate and mouse development demonstrates rare and common variants involved in human malformations. Mol Genet Genomic Med. 2014 Mar;2(2):138-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6449684
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
6A:
embryonic day 12.5
(more )
Specimen
6A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6B:
(close )
Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: palatal shelf epithelium |
Present |
Regionally restricted |
6B
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Expression is seen in the epithelium by the thickened rugae; there is one fewer ruga in the mutant.
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Specimen
6C:
embryonic day 13.0
(more )
Specimen
6C:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6D:
(close )
Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS21: palatal shelf epithelium |
Present |
Regionally restricted |
6D
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Expression is seen in the epithelium by the thickened rugae; there is one fewer ruga in the mutant.
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Specimen
6E:
embryonic day 13.5
(more )
Specimen
6E:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6F:
(close )
Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: palatal shelf epithelium |
Present |
Regionally restricted |
6F
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Expression is seen in the epithelium by the thickened rugae; there is one fewer ruga in the mutant.
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Specimen
6G:
embryonic day 15.0
(more )
Specimen
6G:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6H:
(close )
Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS23: palatal shelf epithelium |
Present |
Regionally restricted |
6H
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Expression is seen in the epithelium by the thickened rugae; at this age the number of ruga in the mutant is the same as in wild type.
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Specimen
7C:
embryonic day 11.5
(more )
Specimen
7C:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: palatal shelf |
Weak |
Regionally restricted |
7C
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Expression is seen in the anterior palate.
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Specimen
7D:
(close )
Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: palatal shelf |
Weak |
Regionally restricted |
7D
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Expression is seen in the anterior palate; in the mutant this domain is 30% shorter than in wild type.
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Specimen
9C:
embryonic day 12.5
(more )
Specimen
9C:
(close )
Genetic Background:
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involves: 101/Rl * C3H/Rl
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9D:
(close )
Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Vangl2Lp-m1Jus/Vangl2Lp-m1Jus
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: palatal shelf |
Present |
Regionally restricted |
9D
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Expression is seen in the epithelium by the thickened rugae; the number of rugae is the same in the mutant as in wild type.
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Specimen
9E:
embryonic day 14.5
(more )
Specimen
9E:
(close )
Genetic Background:
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involves: 101/Rl * C3H/Rl
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9F:
(close )
Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Vangl2Lp-m1Jus/Vangl2Lp-m1Jus
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS22: palatal shelf epithelium |
Present |
Regionally restricted |
9F
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Expression is seen in the epithelium by the thickened rugae; the number of rugae is the same in the mutant as in wild type (although the palate is shorter in the mutant).
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