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Gene Expression Data
Assay Details
Assay
Reference: J:231833 Hashimoto R, et al., Origins of oligodendrocytes in the cerebellum, whose development is controlled by the transcription factor, Sox9. Mech Dev. 2016 May;140:25-40
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6444829
Gene symbol: Olig2
Gene name: oligodendrocyte transcription factor 2
Antibody: anti-Olig2
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-Olig2 MGI:6444794 may have been used in this assay.
Results
Specimen 2A/B: embryonic day 11.5 (more )
Note: Triple labeled: green - Olig2; red - Lmx1a (assay MGI:6444831); blue - DAPI.
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 2B , 2A Expression in portion of neuroepithelium in ventral rhombomere 1 (r1).
TS19: midbrain Absent 2A , 2B
Specimen 2C/D: embryonic day 11.5 (more )
Note: Triple labeled: green - Olig2; red - Lmx1a (assay MGI:6444831); blue - DAPI.
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 2C , 2D Expression in portion of neuroepithelium in ventral rhombomere 1 (r1).
TS19: midbrain Absent 2D , 2C
TS19: cerebellum primordium Present Regionally restricted 2C , 2D Expression in cerebellar ventricular zone.
Specimen 2E: embryonic day 11.5 (more )
Note: Double labeled: green - Olig2; blue - DAPI.
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 2E Expression in neuroepithelial (NE) domain in ventral rhombomere 1 (r1).
Specimen 2F/H: embryonic day 11.5 (more )
Note: Double labeled: green - Olig2; red - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 2F , 2H Expression in oligodendrocyte progenitor cells (OPC) in ventral rhombomere 1 (r1).
Specimen 3E: embryonic day 11.5 (more )
Note: Triple labeled: green - Olig2; pink - Sox9 (assay MGI:6444832); white - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 3E Expression neuroepithelial (NE) domain in ventral rhombomere 1 (r1).
Specimen 3G: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 3G Expression in ventral rhombomere 1 (r1) in oligodendrocyte progenitor cells in mantle zone in vicinity of Olig2 neuroepithelial (NE) domain.
Specimen 4K En1-Cre; Sox9+/+: embryonic day 18.5; En1tm2(cre)Wrst/En1+ (more )
Structure Level Pattern Image Note
TS26: deep cerebellar nucleus Present Regionally restricted 4K En1-Cre: Sox9+/+ Expression in cerebellar nuclei neurons.
Specimen 4K En1-Cre; Sox9flox/flox: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: deep cerebellar nucleus Present Regionally restricted 4K En1-Cre; Sox9flox/flox Number of expressing cerebellar nuclei neurons was not affected in mutant.
Specimen 5J: embryonic day 16.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS24: cerebellum Ambiguous 5J
Specimen 5K: embryonic day 16.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS24: cerebellum Present 5K Authors report that process of oligodendrocytes were short and poorly arborized in cerebella of mutant embryos is a gene dosage dependent manner.
Specimen 5L: embryonic day 16.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS24: cerebellum Present 5L Authors report that process of oligodendrocytes were short and poorly arborized in cerebella of mutant embryos is a gene dosage dependent manner.
Specimen 5M: embryonic day 18.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS26: cerebellum Ambiguous 5M
Specimen 5N: embryonic day 18.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS26: cerebellum Present 5N Authors report that process of oligodendrocytes were short and poorly arborized in cerebella of mutant embryos is a gene dosage dependent manner.
Specimen 5O: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS26: cerebellum Ambiguous 5O Authors report that process of oligodendrocytes were short and poorly arborized in cerebella of mutant embryos is a gene dosage dependent manner.
Specimen 6A: embryonic day 11.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Present Regionally restricted 6A Expression in ventral rhombomere 1 (r1) in discrete neuroepithelial (NE) region facing ventricle.
Specimen 6B: embryonic day 11.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Trace Regionally restricted 6B Dramatic decrease in expressing cells in heterozygous mutants.
Specimen 6C: embryonic day 11.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS19: metencephalon basal plate Absent 6C Expression almost completely absent in homozygous mutants.
Specimen 6D: embryonic day 12.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Present 6D
Specimen 6E: embryonic day 12.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Present 6E Expression in neuroepithelial (NE) region increased to similar extent as in controls.
Specimen 6F: embryonic day 12.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Absent 6F Expression not detecteable in corresponding region.
Specimen 6G: embryonic day 14.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present 6G
Specimen 6H: embryonic day 14.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present 6H
Specimen 6I: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present 6I
Specimen 6J: embryonic day 16.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 6J
Specimen 6K: embryonic day 16.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 6K
Specimen 6L: embryonic day 16.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 6L
Specimen 6M: embryonic day 18.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 6M
Specimen 6N: embryonic day 18.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 6N
Specimen 6O: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Pdgfra (assay MGI:6444828).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 6O
Specimen 7A: embryonic day 12.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - Mki67 (assay MGI:6444842).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Present Regionally restricted 7A Expression in proliferating oligodendrocytes.
Specimen 7B: embryonic day 12.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Mki67 (assay MGI:6444842).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Present Regionally restricted 7B Expression in proliferating oligodendrocytes.
Specimen 7C: embryonic day 12.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Mki67 (assay MGI:6444842).
Structure Level Pattern Image Note
TS20: metencephalon basal plate Absent 7C
Specimen 7D: embryonic day 18.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - Mki67 (assay MGI:6444842).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present Regionally restricted 7D Expression in proliferating oligodendrocytes.
Specimen 7E: embryonic day 18.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Mki67 (assay MGI:6444842).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present Regionally restricted 7E Expression in proliferating oligodendrocytes.
Specimen 7F: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - Mki67 (assay MGI:6444842).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present Regionally restricted 7F Expression in proliferating oligodendrocytes.
Specimen 7K: embryonic day 14.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present 7K
Specimen 7L: embryonic day 14.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present 7L
Specimen 7M: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS22: metencephalon basal plate Present 7M
Specimen 7N: embryonic day 16.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 7N
Specimen 7O: embryonic day 16.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 7O
Specimen 7P: embryonic day 16.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS24: metencephalon basal plate Present 7P
Specimen 7Q: embryonic day 18.5; En1tm2(cre)Wrst/En1+ (more )
Note: Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 7Q
Specimen 7R: embryonic day 18.5; Sox9tm2Crm/Sox9+, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 7R
Specimen 7S: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: green - Olig2; pink - NG2 (Cspg4 assay MGI:6444839).
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 7S
Specimen 8C Olig2: embryonic day 18.5; Sox9tm2Crm/Sox9tm2Crm, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: metencephalon basal plate Present 8C Olig2
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