Opn1mw Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:193822 Lumayag S, et al., Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration. Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):E507-16
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6444824
Gene symbol:	Opn1mw
Gene name:	opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan)

Antibody:	anti-red/green Opsin (#AB5405)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

8A miR-183C^+/+ M opsin ©

8A miR-183C^+/+ Merged ©

Specimen 8A miR-183C^+/+: postnatal week 5 (more

)
Note: Double labeled: green - Opn1mw; red - peanut agglutinin.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8A miR-183C^+/+ M opsin , 8A miR-183C^+/+ Merged	Expression was detected in cones.

8A miR-183C^GT/GT M opsin ©

8A miR-183C^GT/GT Merged ©

Specimen 8A miR-183C^GT/GT: postnatal week 5; Mirc35hg^{Gt(D016D06)Wrst}/Mirc35hg^{Gt(D016D06)Wrst} (more

)
Note: Double labeled: green - Opn1mw; red - peanut agglutinin.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8A miR-183C^GT/GT Merged , 8A miR-183C^GT/GT M opsin	Expression was decreased in the retina of mutant mice. Both the total number and the percentage of M opsin-positive cones in total cones (PNA-positive) were decreased significantly in the superior retina, by 49% and 26%, respectively.

8B Sup. miR-183C^+/+ M opsin ©

8B Sup. miR-183C^+/+ Merged ©

Specimen 8B Sup. miR-183C^+/+: postnatal week 5 (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8B Sup. miR-183C^+/+ M opsin , 8B Sup. miR-183C^+/+ Merged	Expression was detected in cones in the superior retina.

8B Sup. miR-183C^GT/GT M opsin ©

8B Sup. miR-183C^GT/GT Merged ©

Specimen 8B Sup. miR-183C^GT/GT: postnatal week 5; Mirc35hg^{Gt(D016D06)Wrst}/Mirc35hg^{Gt(D016D06)Wrst} (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8B Sup. miR-183C^GT/GT M opsin , 8B Sup. miR-183C^GT/GT Merged	Expression was significantly decreased in the superior retina of the mutant.

8B Inf. miR-183C^+/+ M opsin ©

8B Inf. miR-183C^+/+ Merged ©

Specimen 8B Inf. miR-183C^+/+: postnatal week 5 (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8B Inf. miR-183C^+/+ Merged , 8B Inf. miR-183C^+/+ M opsin	Expression was detected in cones in the inferior retina.

8B Inf. miR-183C^GT/GT M opsin ©

8B Inf. miR-183C^GT/GT Merged ©

Specimen 8B Inf. miR-183C^GT/GT: postnatal week 5; Mirc35hg^{Gt(D016D06)Wrst}/Mirc35hg^{Gt(D016D06)Wrst} (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8B Inf. miR-183C^GT/GT Merged , 8B Inf. miR-183C^GT/GT M opsin	No significant changes were detected in the number of M opsin-positive cones in the inferior retina of the mutant.

8C Sup. miR-183C^+/+ M opsin ©

8C Sup. miR-183C^+/+ Merged ©

Specimen 8C Sup. miR-183C^+/+: postnatal year 1 (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8C Sup. miR-183C^+/+ M opsin , 8C Sup. miR-183C^+/+ Merged	Expression was detected in cones in the superior retina.

Specimen 8C Sup. miR-183C^GT/GT: postnatal year 1; Mirc35hg^{Gt(D016D06)Wrst}/Mirc35hg^{Gt(D016D06)Wrst} (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Trace		8C Sup. miR-183C^GT/GT M opsin , 8C Sup. miR-183C^GT/GT Merged	At 1 year, M opsin was almost complete lost in the mutant retina.

Specimen 8C Inf. miR-183C^+/+: postnatal year 1 (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8C Inf. miR-183C^+/+ Merged , 8C Inf. miR-183C^+/+ M opsin	Expression was detected in cones in the inferior retina.

Specimen 8C Inf. miR-183C^GT/GT: postnatal year 1; Mirc35hg^{Gt(D016D06)Wrst}/Mirc35hg^{Gt(D016D06)Wrst} (more

)
Note: Double labeled: red - Opn1mw; green - Rho (assay MGI:6444826).

Structure	Level	Pattern	Image	Note
TS28: retina	Trace		8C Inf. miR-183C^GT/GT Merged , 8C Inf. miR-183C^GT/GT M opsin	At 1 year, M opsin was almost complete lost in the mutant retina.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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