Hes1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199297 Kitagawa M, et al., Hes1 and Hes5 regulate vascular remodeling and arterial specification of endothelial cells in brain vascular development. Mech Dev. 2013 Sep-Oct;130(9-10):458-66
Assay type:	RNA in situ
MGI Accession ID:	MGI:6441782
Gene symbol:	Hes1
Gene name:	hes family bHLH transcription factor 1

Probe:	Hes1 probe20
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Fixation was with 0.2% glutaraldehyde/4% paraformaldehyde.

Results

1A ©

Specimen 1A: embryonic day 10.5; Hes1^tm1Kag/Hes1⁺, Hes5^tm1Fgu/Hes5^tm1Fgu (more

)

Structure	Level	Pattern	Image	Note
TS17: internal carotid artery	Present		1A	Expression in almost all endothelial cells of the developing internal carotid artery at E10.5.

2A ©

Specimen 2A: embryonic day 10.5; Hes1^tm1Kag/Hes1⁺, Hes5^tm1Fgu/Hes5^tm1Fgu (more

)

Structure	Level	Pattern	Image	Note
TS17: internal carotid artery	Present	Regionally restricted	2A	Expression in endothelial cells.
TS17: central nervous system	Present		2A	Expression in neural tube.

2B ©

Specimen 2B: embryonic day 10.5; Hes1^tm1Fgu/Hes1^tm1Kag, Hes5^tm1Fgu/Hes5^tm1Fgu, Tg(Tek-cre)12Flv/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: internal carotid artery	Absent		2B	No expression in endothelial cells of internal carotid artery.
TS17: central nervous system	Present		2B	Normal expression in neural tube.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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