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Gene Expression Data
Assay Details
Assay
Reference: J:229352 Metzger DE, et al., Grg3/TLE3 and Grg1/TLE1 induce monohormonal pancreatic beta-cells while repressing alpha-cell functions. Diabetes. 2014 May;63(5):1804-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6441475
Gene symbol: Ins2
Gene name: insulin II
Antibody: anti-insulin
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 1A: embryonic day 17.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Tle3 (assay MGI:6441177); gray - Gcg (assay MGI:6441170).
Structure Level Pattern Image Note
TS25: pancreas Present 1A right , 1A middle The majority of insulin-positive b-cells expressed Tle3. Many Gcg-positive alpha-cells were Tle3 negative or had low-level nuclear Tle3 expression.
Specimen 2A +/+: embryonic day 17.5 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Gcg (assay MGI:6441171).
Structure Level Pattern Image Note
TS25: pancreas Present 2A left Expressed in beta-cells.
Specimen 2A +/-: embryonic day 17.5; Tle3tm1Jcc/Tle3+ (more )
Note: Double labeled: red - Ins1 and Ins2; green - Gcg (assay MGI:6441171).
Structure Level Pattern Image Note
TS25: pancreas Present 2A right An increase in bihormonal insulin-positive/glucagon-positive cells. Decreased number of Insulin+ beta-cells.
Specimen 2C +/+: postnatal adult (more )
Note: Double labeled: red - Ins1 and Ins2; green - Gcg (assay MGI:6441171).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 2C left Expressed in beta-cells.
Specimen 2C +/-: postnatal adult; Tle3tm1Jcc/Tle3+ (more )
Note: Double labeled: red - Ins1 and Ins2; green - Gcg (assay MGI:6441171).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 2C right An increase in bihormonal insulin-positive/glucagon-positive cells. Decreased number of Insulin+ beta-cells.
Specimen S1A: embryonic day 17.5 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Tle1 (assay MGI:6441185).
Structure Level Pattern Image Note
TS25: pancreas Present Regionally restricted S1A right , S1A left Expressed in beta-cells. Tle1 did not stain the insulin+ cells.
Specimen S1B: postnatal newborn (more )
Note: Double labeled: red - Ins1 and Ins2; green - Tle1 (assay MGI:6441185).
Structure Level Pattern Image Note
TS27: pancreas Present Regionally restricted S1B left , S1B right Expressed in beta-cells. Tle1 did not stain the insulin+ cells.
Specimen S1C: postnatal adult (more )
Note: Double labeled: red - Ins1 and Ins2; green - Tle1 (assay MGI:6441185).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present S1C left , S1C right Tle1 was robustly expressed in adult mouse islets.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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