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Gene Expression Data
Assay Details
Assay
Reference: J:287370 Chen W, et al., Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy. Elife. 2020 Feb 19;9:e48705
Assay type: Western blot
MGI Accession ID: MGI:6437215
Gene symbol: Stxbp1
Gene name: syntaxin binding protein 1
Antibody: anti-Stxbp1 (abcam; ab3451)
Detection system: Secondary antibody coupled to other - see notes
Assay notes: The authors did not specify which antibody was used to obtain these data; it is possible MGI:6437135 was used instead. The antibody was detected using a secondary conjugated with IRDye 680LT, allowing for visualization and quantification, which is presented in part C of the figure.
Results
Image: 1B tm1d ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
aCTX P m 3 TS28: cerebral cortex Present C57BL/6 Not Specified anterior cortex
aCTX P m 3 TS28: cerebral cortex Present (a) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified anterior cortex
pCTX P m 3 TS28: cerebral cortex Present C57BL/6 Not Specified posterior cortex
pCTX P m 3 TS28: cerebral cortex Present (a) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified posterior cortex
HIP P m 3 TS28: hippocampus Present C57BL/6 Not Specified
HIP P m 3 TS28: hippocampus Present (a) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified
TH+HY P m 3 TS28: hypothalamus
TS28: thalamus 		Present C57BL/6 Not Specified
TH+HY P m 3 TS28: hypothalamus
TS28: thalamus 		Present (a) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified
STR P m 3 TS28: corpus striatum Present C57BL/6 Not Specified
STR P m 3 TS28: corpus striatum Present (a) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified
CB P m 3 TS28: cerebellum Present C57BL/6 Not Specified
CB P m 3 TS28: cerebellum Present (b) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified
OLF P m 3 TS28: main olfactory bulb Present C57BL/6 Not Specified
OLF P m 3 TS28: main olfactory bulb Present (b) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified
MB+HB P m 3 TS28: hindbrain
TS28: midbrain 		Present C57BL/6 Not Specified
MB+HB P m 3 TS28: hindbrain
TS28: midbrain 		Present (a) B6(Cg)-Stxbp1tm1d(EUCOMM)Hmgu Stxbp1tm1d(EUCOMM)Hmgu/Stxbp1+Not Specified
Notes:
(a) Expression levels are reduced 40-50% compared to wild type.
(b) Expression levels are reduced 20-30% compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory