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Gene Expression Data
Assay Details
Assay
Reference: J:268273 Simon CS, et al., A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse. Biol Open. 2018 Dec 10;7(12):bio036517
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6434233
Gene symbol: Gata4
Gene name: GATA binding protein 4
Antibody: anti-GATA-4 (sc-9053)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2A E3.5: embryonic day 3.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early stage blastocyst (61 cells). Double labeled: blue - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2A E3.5 Merge , 2A E3.5 GATA4 , 2A E3.5 Merge DNA
Specimen 2A E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (76 cells). Double labeled: blue - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A E3.75 GATA4 , 2A E3.75 Merge , 2A E3.75 Merge DNA
Specimen 2A E4.0: embryonic day 4.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (110 cells). Double labeled: blue - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A E4.0 GATA4 , 2A E4.0 Merge , 2A E4.0 Merge DNA
Specimen 2A E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (141 cells). Double labeled: blue - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2A E4.5 Merge DNA , 2A E4.5 GATA4 , 2A E4.5 Merge
Specimen 3A top: embryonic day 5.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3A MergeDNA top , 3A GFP/GATA4 top , 3A GATA4 top
TS8: primitive endoderm Present 3A MergeDNA top , 3A GFP/GATA4 top , 3A GATA4 top
TS8: epiblast Absent 3A GATA4 top , 3A MergeDNA top , 3A GFP/GATA4 top
TS8: extraembryonic ectoderm Absent 3A GATA4 top , 3A MergeDNA top , 3A GFP/GATA4 top
Specimen 3A bottom: embryonic day 5.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3A GATA4 bottom , 3A MergeDNA bottom , 3A GFP/GATA4 bottom
TS8: primitive endoderm Present 3A GATA4 bottom , 3A MergeDNA bottom , 3A GFP/GATA4 bottom
TS8: epiblast Absent 3A MergeDNA bottom , 3A GATA4 bottom , 3A GFP/GATA4 bottom
TS8: extraembryonic ectoderm Absent 3A MergeDNA bottom , 3A GATA4 bottom , 3A GFP/GATA4 bottom
Specimen 3B top: embryonic day 6.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Pre-streak stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3B GATA4 top , 3B GFP/GATA4 top , 3B MergeDNA top
TS8: primitive endoderm Present 3B GATA4 top , 3B GFP/GATA4 top , 3B MergeDNA top
TS8: epiblast Absent 3B GATA4 top , 3B MergeDNA top , 3B GFP/GATA4 top
TS8: extraembryonic ectoderm Absent 3B GATA4 top , 3B MergeDNA top , 3B GFP/GATA4 top
Specimen 3B bottom: embryonic day 6.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Pre-streak stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3B GFP/GATA4 bottom , 3B GATA4 bottom , 3B MergeDNA bottom
TS8: primitive endoderm Present 3B GFP/GATA4 bottom , 3B GATA4 bottom , 3B MergeDNA bottom
TS8: epiblast Absent 3B MergeDNA bottom , 3B GFP/GATA4 bottom , 3B GATA4 bottom
TS8: extraembryonic ectoderm Absent 3B MergeDNA bottom , 3B GFP/GATA4 bottom , 3B GATA4 bottom
Specimen 4A top: embryonic day 6.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-streak stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 4A Merge top , 4A GFP/GATA4 top , 4A GATA4 top
TS9: primitive endoderm Present 4A Merge top , 4A GFP/GATA4 top , 4A GATA4 top
Specimen 4A bottom: embryonic day 6.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-streak stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 4A GFP/GATA4 bottom , 4A GATA4 bottom , 4A Merge bottom
TS9: primitive endoderm Present 4A GFP/GATA4 bottom , 4A GATA4 bottom , 4A Merge bottom
Specimen 4B top: embryonic day 7.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early bud stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS11: primitive endoderm Present Regionally restricted 4B Merge top , 4B GATA4 top , 4B GFP/GATA4 top Expression is sparse overlying the epiblast.
TS11: embryo mesoderm Present 4B Merge top , 4B GFP/GATA4 top , 4B GATA4 top
Specimen 4B bottom: embryonic day 7.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early bud stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS11: primitive endoderm Present Regionally restricted 4B Merge bottom , 4B GFP/GATA4 bottom , 4B GATA4 bottom Expression is sparse overlying the epiblast.
TS11: embryo mesoderm Present 4B GFP/GATA4 bottom , 4B Merge bottom , 4B GATA4 bottom
Specimen 5a: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS13: primitive ventricle Present Regionally restricted 5a MergeDNA , 5a GFP/GATA4 , 5a GATA4 Expression is in both the outer myocardium and the inner endocardium and is lower in the region separating the left and right ventricles.
TS13: primitive ventricle cardiac muscle Present 5a GATA4 , 5a MergeDNA , 5a GFP/GATA4
Specimen 5b: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS13: primitive ventricle Present Regionally restricted 5b MergeDNA , 5b GFP/GATA4 , 5b GATA4 Expression is in both the outer myocardium and the inner endocardium.
TS13: primitive ventricle cardiac muscle Present 5b GFP/GATA4 , 5b MergeDNA , 5b GATA4
TS13: septum transversum Present 5b GFP/GATA4 , 5b MergeDNA , 5b GATA4
TS13: sinus venosus Present 5b GFP/GATA4 , 5b MergeDNA , 5b GATA4
TS13: midgut endoderm Present Regionally restricted 5b GFP/GATA4 , 5b GATA4 , 5b MergeDNA Expression is in the anterior midgut.
TS13: yolk sac endoderm Present 5b MergeDNA
TS13: foregut diverticulum endoderm Absent 5b MergeDNA
TS13: hindgut diverticulum endoderm Absent 5b MergeDNA
Specimen S3A top: embryonic day 6.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early streak stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present S3A GFP/GATA4 top , S3A GATA4 top , S3A MergeDNA top
TS9: primitive endoderm Present S3A GFP/GATA4 top , S3A GATA4 top , S3A MergeDNA top
Specimen S3A bottom: embryonic day 6.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early streak stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present S3A MergeDNA bottom , S3A GFP/GATA4 bottom , S3A GATA4 bottom
TS9: primitive endoderm Present S3A MergeDNA bottom , S3A GFP/GATA4 bottom , S3A GATA4 bottom
Specimen S3B top: embryonic day 7.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early head fold stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS12: primitive endoderm Present Regionally restricted S3B GATA4 top , S3B MergeDNA top , S3B GFP/GATA4 top Expression is sparse overlying the epiblast.
TS12: embryo mesoderm Present Regionally restricted S3B GFP/GATA4 top , S3B GATA4 top , S3B MergeDNA top Expression is in the mesoderm layer at the proximal-anterior region of the embryo.
Specimen S3B bottom: embryonic day 7.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early head fold stage embryo. Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS12: primitive endoderm Present Regionally restricted S3B GATA4 bottom , S3B MergeDNA bottom , S3B GFP/GATA4 bottom Expression is sparse overlying the epiblast.
TS12: embryo mesoderm Present Regionally restricted S3B GATA4 bottom , S3B MergeDNA bottom , S3B GFP/GATA4 bottom Expression is in the mesoderm layer at the proximal-anterior region of the embryo.
Specimen S4: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: red - Gata4 protein; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS13: yolk sac Present S4_GFP/GATA4 , S4_MergeDNA , S4_GATA4
TS13: midgut Present Regionally restricted S4_GFP/GATA4 , S4_GATA4 , S4_MergeDNA Expression is in the anterior midgut and is absent in the posterior midgut.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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