About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:192194 Tortelote GG, et al., Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice. Dev Biol. 2013 Feb 1;374(1):164-73
Assay type: RNA in situ
MGI Accession ID: MGI:6430585
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Probe: T cDNA9
Probe preparation: labelled with digoxigenin RNA
Results
Specimen 1D: embryonic day 6.5; Wnt3tm2Amc/Wnt3+, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Present Regionally restricted 1D Expression in posterior epiblast.
TS9: extraembryonic ectoderm Present 1D
Specimen 1D Epi KO: embryonic day 6.5; Wnt3tm1Bhr/Wnt3tm2Amc, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Absent 1D Epi KO Expression in posterior epiblast was absent in 7 of 10 mutant embryos examined; weak expression was seen in the other 3.
TS9: extraembryonic ectoderm Weak 1D Epi KO Reduced expression in extraembryonic ectoderm.
Specimen S2 (left): embryonic day 6.5; Wnt3tm2Amc/Wnt3+, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Present S2_(left)
TS9: extraembryonic ectoderm Present S2_(left)
Specimen S2 (center): embryonic day 6.5; Wnt3tm2Amc/Wnt3+, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Present S2_(center)
TS9: extraembryonic ectoderm Present S2_(center)
Specimen S2 (right): embryonic day 6.5; Wnt3tm2Amc/Wnt3+, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Present S2_(right)
TS9: extraembryonic ectoderm Present S2_(right)
Specimen S2 Epi KO (left): embryonic day 6.5; Wnt3tm1Bhr/Wnt3tm2Amc, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Weak Regionally restricted S2_Epi KO (left) Weak expression detected in posterior epiblast in 3 of 10 mutant embryos; no expression was seen in the other 7.
TS9: extraembryonic ectoderm Weak S2_Epi KO (left) Expression in extraembryonic ectoderm weaker than in control embryos.
Specimen S2 Epi KO (right): embryonic day 6.5; Wnt3tm1Bhr/Wnt3tm2Amc, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Weak Regionally restricted S2_Epi KO (right) Weak expression detected in posterior epiblast in 3 of 10 mutant embryos; no expression was seen in the other 7.
TS9: extraembryonic ectoderm Weak S2_Epi KO (right) Weaker expression in extraembryonic ectoderm than in control embryos.
Specimen S2 (far right): embryonic day 6.5; Wnt3tm2Amc/Wnt3+, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Present S2_(far right)
TS9: extraembryonic ectoderm Present S2_(far right)
Specimen 2A: embryonic day 7.5; Wnt3tm2Amc/Wnt3+, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: primitive streak Present 2A Expression in axial midline region.
Specimen 2A Epi KO (left): embryonic day 7.5; Wnt3tm1Bhr/Wnt3tm2Amc, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: primitive streak Present Regionally restricted 2A Epi KO (left) Axial midline failed to extend to same rostral levels as control embryos.
Specimen 2A Epi KO (center): embryonic day 7.5; Wnt3tm1Bhr/Wnt3tm2Amc, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: primitive streak Present Regionally restricted 2A Epi KO (center) Axial midline failed to extend to same rostral levels as control embryos.
Specimen 2A Epi KO (right): embryonic day 7.5; Wnt3tm1Bhr/Wnt3tm2Amc, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: primitive streak Present 2A Epi KO (right)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/30/2025
MGI 6.24 		The Jackson Laboratory