Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:285730
Major AT, et al., Dynamic paraspeckle component localisation during spermatogenesis. Reproduction. 2019 Sep;158(3):267-280
|
|
Assay type:
|
Northern blot
|
|
MGI Accession ID:
|
MGI:6430492
|
|
Gene symbol:
|
Neat1
|
|
Gene name:
|
nuclear paraspeckle assembly transcript 1 (non-protein coding)
|
|
Probe:
|
Neat1 ISH probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The probe used in this this assay was designed to recognize both the long and short transcripts. However, the long transcript (20.8 kb) was not observed on this blot. It was unclear whether this was due to its absence, to the probe design or due to its inability to transfer to the membrane (due to size).
|
|
|
Results
|
Image: 5B
| P adult
|
TS28: testis
|
Present
|
30 µg; total RNA |
involves: C57BL/6 * CBA * Swiss |
| Male |
| P adult
|
TS28: metanephros
|
Present
|
30 µg; total RNA |
involves: C57BL/6 * CBA * Swiss |
| Not Specified |
| P adult
|
TS28: liver
|
Present
|
30 µg; total RNA |
involves: C57BL/6 * CBA * Swiss |
| Not Specified |
Notes:
|
Analysis Tools
