Probe:	Chrd cDNA3
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS13: notochord	Strong		1A

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		1B
TS17: forebrain	Present		1B
TS17: hindbrain	Present		1B
TS17: liver	Present		1B
TS17: lung	Present		1B
TS17: midbrain	Present		1B
TS17: neural tube	Present		1B
TS17: embryo mesenchyme	Weak		1B
TS17: heart	Weak		1B
TS17: limb	Weak		1B

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Present		1C
TS19: forebrain	Present		1C
TS19: hindbrain	Present		1C
TS19: liver	Present		1C
TS19: lung	Present		1C
TS19: midbrain	Present		1C
TS19: neural tube	Present		1C
TS19: vertebral cartilage condensation	Present		1C
TS19: embryo mesenchyme	Weak		1C
TS19: heart	Weak		1C
TS19: limb	Weak		1C

Structure	Level	Pattern	Image	Note
TS20: spinal cord marginal layer	Present		1D
TS20: vertebral cartilage condensation	Present		1D
TS20: tongue	Present	Regionally restricted	1D	Expression is in the dorsal regions.
TS20: embryo mesenchyme	Weak		1D

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		1E
TS21: axial skeleton tail region	Present	Regionally restricted	1E	Expression is in the perichondria surrounding vertebral elements of the tail.
TS21: rib cartilage condensation	Weak	Regionally restricted	1E	Expression is in the perichondria of the ribs.
TS21: femur cartilage condensation	Weak	Regionally restricted	1E	Expression is in the perichondria of the femoral head.
TS21: perichondrium	Present	Regionally restricted	1E	Expression is in the perichondria surrounding vertebral elements of the tail and is weak in the perichondria of the ribs and in the perichondria of the femoral head.
TS21: embryo mesenchyme	Weak		1E
TS21: heart ventricle wall	Weak		1E
TS21: liver	Weak		1E
TS21: lung	Weak		1E
TS21: metanephros	Weak		1E

Structure	Level	Pattern	Image	Note
TS23: hindlimb skeleton	Strong		1F
TS23: perichondrium	Strong	Regionally restricted	1F	Expression is in the perichondria of the tarsals, metatarsals, phalanges, femur, vertebrae, ribs, basioccipital, and basisphenoid bones.
TS23: femur	Strong	Regionally restricted	1F	Expression is in the perichondria.
TS23: hindlimb phalanx	Strong	Regionally restricted	1F	Expression is in the perichondria.
TS23: metatarsus	Strong	Regionally restricted	1F	Expression is in the perichondria.
TS23: tarsal bone	Strong	Regionally restricted	1F	Expression is in the perichondria.
TS23: cerebellum	Strong	Regionally restricted	1F	Expression is in the neuroepithelium.
TS23: forebrain	Strong	Regionally restricted	1F	Expression is in the neuroepithelium.
TS23: midbrain	Strong	Regionally restricted	1F	Expression is in the neuroepithelium.
TS23: spinal cord mantle layer	Strong	Regionally restricted	1F	Expression is in the neuroepithelium.
TS23: basioccipital bone	Present	Regionally restricted	1F	Expression is in the perichondrium/periosteum.
TS23: basisphenoid bone	Present	Regionally restricted	1F	Expression is in the perichondrium/periosteum.
TS23: rib	Present	Regionally restricted	1F	Expression is in the perichondrium/periosteum.
TS23: vertebra	Present	Regionally restricted	1F	Expression is in the perichondrium/periosteum.
TS23: periosteum	Present	Regionally restricted	1F	Expression is in the periosteum of the vertebrae, ribs, basioccipital, and basisphenoid bones.
TS23: gut mucosa	Present		1F
TS23: liver	Present		1F
TS23: metanephros	Present		1F
TS23: submandibular gland	Present		1F
TS23: thymus primordium	Present		1F
TS23: tongue	Present		1F
TS23: vibrissa follicle	Present		1F
TS23: embryo mesenchyme	Weak		1F

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Weak		2A

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Weak	Regionally restricted	2B	Expression is in condensing mesenchyme at the distal tips of the digits.
TS21: hindlimb digit mesenchyme	Weak	Regionally restricted	2B	Expression is in condensing mesenchyme at the distal tips of the digits.

Structure	Level	Pattern	Image	Note
TS23: hindlimb skeleton	Strong		2C
TS23: perichondrium	Strong	Regionally restricted	2C	Expression is in the perichondria of the tarsals, metatarsals, phalanges, and femur.
TS23: hindlimb phalanx	Strong	Regionally restricted	2C	Expression is in the perichondria.
TS23: metatarsus	Strong	Regionally restricted	2C	Expression is in the perichondria.
TS23: tarsal bone	Strong	Regionally restricted	2C	Expression is in the perichondria.
TS23: hindlimb digit	Strong	Regionally restricted	2C	Exression is at the tips.
TS23: hindlimb digit 1 interphalangeal joint	Strong	Regionally restricted	2C	Expression is in the joint-forming cavities of each digit.
TS23: hindlimb digit 2 interphalangeal joint	Strong	Regionally restricted	2C	Expression is in the joint-forming cavities of each digit.
TS23: hindlimb digit 3 interphalangeal joint	Strong	Regionally restricted	2C	Expression is in the joint-forming cavities of each digit.
TS23: hindlimb digit 4 interphalangeal joint	Strong	Regionally restricted	2C	Expression is in the joint-forming cavities of each digit.
TS23: hindlimb digit 5 interphalangeal joint	Strong	Regionally restricted	2C	Expression is in the joint-forming cavities of each digit.

Structure	Level	Pattern	Image	Note
TS23: lung	Present	Regionally restricted	2D	Expression is in the mesoderm surrounding the major bronchi and trachea.

Structure	Level	Pattern	Image	Note
TS23: submandibular gland	Present		2E
TS23: thymus primordium	Present		2E
TS23: tongue	Present		2E
TS23: trachea	Present		2E
TS23: perichondrium	Present	Regionally restricted	2E	Expression is in the perichondrium/periosteum of the vertebrae, ribs, basioccipital, and basisphenoid bones.
TS23: periosteum	Present	Regionally restricted	2E	Expression is in the perichondrium/periosteum of the vertebrae, ribs, basioccipital, and basisphenoid bones.
TS23: vertebra	Present	Regionally restricted	2E	Expression is in the perichondrium/periosteum of the vertebrae.
TS23: rib	Present	Regionally restricted	2E	Expression is in the perichondrium/periosteum of the ribs.
TS23: basioccipital bone	Present	Regionally restricted	2E	Expression is in the perichondrium/periosteum of the basioccipital bone.
TS23: basisphenoid bone	Present	Regionally restricted	2E	Expression is in the perichondrium/periosteum of the basisphenoid bone.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		3A
TS28: dentate gyrus	Strong		3A
TS28: hippocampus CA1	Strong		3A
TS28: hippocampus CA2	Strong		3A
TS28: hippocampus CA3	Strong		3A
TS28: cerebral cortex	Weak		3A
TS28: inferior colliculus	Weak		3A
TS28: superior colliculus	Weak		3A

Structure	Level	Pattern	Image	Note
TS15: embryo	Trace