Gene Expression Data
Assay Details
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Assay
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Reference:
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J:161404
Stevens J, et al., Analysis of the asymmetrically expressed Ablim1 locus reveals existence of a lateral plate Nodal-independent left sided signal and an early, left-right independent role for nodal flow. BMC Dev Biol. 2010;10:54
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6415017
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Gene symbol:
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Ablim1
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Gene name:
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actin-binding LIM protein 1
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Results
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Specimen
1f:
embryonic day 8.5
(more  )
Note: flat mounted
Specimen
1f:
(close  )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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flat mounted
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: heart |
Present |
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1f
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Symmetric expression.
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Specimen
1j:
embryonic day 8.5
(more )
Note: embryos within yolk sacs
Specimen
1j:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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embryos within yolk sacs
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
Regionally restricted |
1j
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Expressed in the right side.
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Specimen
4a:
embryonic day 7.5
(more )
Note: early headfold stage
Specimen
4a:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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early headfold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
Punctate |
4a
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A salt and pepper pattern stretching across the pit of the node.
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Specimen
4b:
embryonic day 7.5
(more )
Note: mid headfold stage
Specimen
4b:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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mid headfold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
Punctate |
4b
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A salt and pepper pattern stretching across the pit of the node.
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Specimen
4c:
embryonic day 7.5
(more )
Note: late headfold stage
Specimen
4c:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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late headfold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
Regionally restricted |
4c
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Expressed as a ring surrounding the node by the mid-headfold stage, with a higher level of expression on the right than the left hand side.
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Specimen
4d:
embryonic day 8.0
(more )
Note: early somite stage
Specimen
4d:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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early somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
4d
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Expression on the anterior left side of the node was lost, while expression was activated in the midline cells anterior to the node, resulting in a question mark-like expression pattern.
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Specimen
4e:
embryonic day 8.0
(more )
Note: 4 somites
Specimen
4e:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
4e
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The remaining left-sided expression was lost, resulting in solely right-sided expression.
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Specimen
4f:
embryonic day 8.0
(more )
Note: 5 somites
Specimen
4f:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
4f
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Abrogation of expression continued in a clockwise direction around the node, resulting in less expression area on the right side.
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Specimen
4g:
embryonic day 8.0
(more )
Note: 7 somites
Specimen
4g:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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7 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
4g
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Expression was restricted to midline cells anterior to the node.
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Specimen
4h:
embryonic day 8.5
(more )
Note: 8 somites
Specimen
4h:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8 somites
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Specimen
4i:
embryonic day 8.0
(more )
Note: late headfold stage
Specimen
4i:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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late headfold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
4i
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Expression was restricted to the ventral layer.
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Specimen
5a:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Patchy |
5a
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The pattern of expression was the same patchy expression seen in the very earliest wild type nodes (summarized in Table 3).
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Specimen
5b:
(close )
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Genetic Background:
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involves: BALB/cAnN
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Mutant Allele(s):
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Pkd2lrm4/Pkd2lrm4
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Patchy |
5b
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Same patchy expression we detected in the embryos lacking nodal flow (summarized in Table 3).
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Specimen
5c:
embryonic day 8.0
(more )
Note: 5 somites
Specimen
5c:
(close )
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Genetic Background:
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(C3H/HeH x 101/H)F1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
5c
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Right-sided expression.
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Analysis Tools