Fgf8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:203431 Collette NM, et al., Sost and its paralog Sostdc1 coordinate digit number in a Gli3-dependent manner. Dev Biol. 2013 Nov 1;383(1):90-105
Assay type:	RNA in situ
MGI Accession ID:	MGI:6406359
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Probe:	Fgf8 probe103
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

6C WT left ©

Specimen 6C WT left: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		6C WT left

6C WT right ©

Specimen 6C WT right: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		6C WT right

6C Sost-/- left ©

Specimen 6C Sost-/- left: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted	6C Sost-/- left	AER expression expanded and disorganized.

6C Sost-/- right ©

Specimen 6C Sost-/- right: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted	6C Sost-/- right	AER expression expanded and disorganized.

6C Sostdc1-/- left ©

Specimen 6C Sostdc1-/- left: embryonic day 10.5; Gli3^Xt-J/Gli3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		6C Sostdc1-/- left

6C Sostdc1-/- right ©

Specimen 6C Sostdc1-/- right: embryonic day 10.5; Gli3^Xt-J/Gli3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		6C Sostdc1-/- right

6C Sost-/-;Sostdc1-/- left ©

Specimen 6C Sost-/-;Sostdc1-/- left: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz, Sostdc1^tm1Myan/Sostdc1^tm1Myan (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted	6C Sost-/-;Sostdc1-/- left	AER expression expanded and disorganized. Expression reduced on anterior side.

6C Sost-/-;Sostdc1-/- right ©

Specimen 6C Sost-/-;Sostdc1-/- right: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz, Sostdc1^tm1Myan/Sostdc1^tm1Myan (more

)

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted	6C Sost-/-;Sostdc1-/- right	AER expression expanded and disorganized. Expression reduced on anterior side.

6F WT ©

Specimen 6F WT: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present		6F WT

6F Sost-/- ©

Specimen 6F Sost-/-: embryonic day 11.5; Sost^tm1Paz/Sost^tm1Paz (more

)

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present	Regionally restricted	6F Sost-/-	AER expression expanded and disorganized.

6F Sostdc1-/- ©

Specimen 6F Sostdc1-/-: embryonic day 11.5; Gli3^Xt-J/Gli3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present		6F Sostdc1-/-

Specimen 6F Sost-/-;Sostdc1-/-: embryonic day 11.5; Sost^tm1Paz/Sost^tm1Paz, Sostdc1^tm1Myan/Sostdc1^tm1Myan (more

)

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present	Regionally restricted	6F Sost-/-;Sostdc1-/-	AER expression expanded and disorganized. Expression reduced on anterior side.

6G WT ©

Specimen 6G WT: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: limb epithelium	Present	Regionally restricted	6G WT

6G Sost-/- ©

Specimen 6G Sost-/-: embryonic day 12.5; Sost^tm1Paz/Sost^tm1Paz (more

)

Structure	Level	Pattern	Image	Note
TS20: limb epithelium	Present	Regionally restricted	6G Sost-/-	AER expression expanded and disorganized.

Specimen 6G Sostdc1-/-: embryonic day 12.5; Gli3^Xt-J/Gli3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: limb epithelium	Present	Regionally restricted	6G Sostdc1-/-

Specimen 6G Sost-/-;Sostdc1-/-: embryonic day 12.5; Sost^tm1Paz/Sost^tm1Paz, Sostdc1^tm1Myan/Sostdc1^tm1Myan (more

)

Structure	Level	Pattern	Image	Note
TS20: limb epithelium	Present	Regionally restricted	6G Sost-/-;Sostdc1-/-	AER expression expanded and disorganized. Expression reduced on anterior side.

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