Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:203431 Collette NM, et al., Sost and its paralog Sostdc1 coordinate digit number in a Gli3-dependent manner. Dev Biol. 2013 Nov 1;383(1):90-105
Assay type:	RNA in situ
MGI Accession ID:	MGI:6406357
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe171
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

6A WT left ©

Specimen 6A WT left: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A WT left

6A WT right ©

Specimen 6A WT right: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A WT right

6A Sost-/- left ©

Specimen 6A Sost-/- left: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A Sost-/- left	Expression domain expanded both anteriorly and distally compared to wild type and Sostdc1-/- limb buds.

6A Sost-/- right ©

Specimen 6A Sost-/- right: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A Sost-/- right	Expression domain expanded both anteriorly and distally compared to wild type and Sostdc1-/- limb buds.

6A Sostdc1-/- left ©

Specimen 6A Sostdc1-/- left: embryonic day 10.5; Gli3^Xt-J/Gli3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A Sostdc1-/- left

6A Sostdc1-/- right ©

Specimen 6A Sostdc1-/- right: embryonic day 10.5; Gli3^Xt-J/Gli3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A Sostdc1-/- right

6A Sost-/-;Sostdc1-/- left ©

Specimen 6A Sost-/-;Sostdc1-/- left: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz, Sostdc1^tm1Myan/Sostdc1^tm1Myan (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A Sost-/-;Sostdc1-/- left	Expression domain expanded compared to wild type along proximal-distal axis of limb bud.

6A Sost-/-;Sostdc1-/- right ©

Specimen 6A Sost-/-;Sostdc1-/- right: embryonic day 10.5; Sost^tm1Paz/Sost^tm1Paz, Sostdc1^tm1Myan/Sostdc1^tm1Myan (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	6A Sost-/-;Sostdc1-/- right	Expression domain expanded compared to wild type slong anterior-posterior axis of limb bud. No ectopic expression in anterior region.

6D WT ©

Specimen 6D WT: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present	Regionally restricted	6D WT

6D Sost-/- ©

Specimen 6D Sost-/-: embryonic day 11.5; Sost^tm1Paz/Sost^tm1Paz (more

)

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present	Regionally restricted	6D Sost-/-	Expression domain expanded compared to wild type and Sostdc1-/- limb buds.

Specimen 6D Sostdc1-/-: embryonic day 11.5; Gli3^Xt-J/Gli3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present	Regionally restricted	6D Sostdc1-/-

Specimen 6D Sost-/-;Sostdc1-/-: embryonic day 11.5; Sost^tm1Paz/Sost^tm1Paz, Sostdc1^tm1Myan/Sostdc1^tm1Myan (more

)

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present	Regionally restricted	6D Sost-/-;Sostdc1-/-	Expression domain expanded compared to wild type and Sostdc1-/- limb buds. No ectopic expression in anterior region.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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