Probe:	3'Nav2
Probe preparation:	Antisense labelled with horseradish peroxidase RNA

Structure	Level	Pattern	Image	Note
TS25: cerebellum granule cell layer	Present		2_E17.5 WT 3'Nav2
TS25: cerebral cortex	Present		2_E17.5 WT 3'Nav2
TS25: hippocampus	Present		2_E17.5 WT 3'Nav2
TS25: main olfactory bulb	Present		2_E17.5 WT 3'Nav2
TS25: thalamus	Present		2_E17.5 WT 3'Nav2
TS25: brain	Present	Non-Uniform	2_E17.5 WT 3'Nav2	Lighter staining was noted throughout many regions of the brain.
TS25: brain white matter	Absent		2_E17.5 WT 3'Nav2
TS25: caudate-putamen	Absent		2_E17.5 WT 3'Nav2

Structure	Level	Pattern	Image	Note
TS25: brain	Trace		2_E17.5 Hyp 3'Nav2	Very little staining was observed.

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present		2_P0 WT 3'Nav2	Expression peaked around birth.
TS27: cerebral cortex	Present		2_P0 WT 3'Nav2	Expression peaked around birth.
TS27: hippocampus	Present		2_P0 WT 3'Nav2	Expression peaked around birth.
TS27: main olfactory bulb	Present		2_P0 WT 3'Nav2	Expression peaked around birth.
TS27: thalamus	Present		2_P0 WT 3'Nav2	Expression peaked around birth.
TS27: brain	Present	Non-Uniform	2_P0 WT 3'Nav2	Lighter staining was noted throughout many regions of the brain.
TS27: brain white matter	Absent		2_P0 WT 3'Nav2
TS27: caudate-putamen	Absent		2_P0 WT 3'Nav2

Structure	Level	Pattern	Image	Note
TS27: brain	Trace		2_P0 Hyp 3'Nav2	Very little staining was observed.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Regionally restricted	2_P7 WT 3'Nav2	With the 3' probe, strongly labeled cells were noted at the base of the cerebellum. As these were not seen with the 5' probe, these represent the shorter transcript.
TS28: cerebellum granule cell layer	Present		2_P7 WT 3'Nav2
TS28: cerebral cortex	Present		2_P7 WT 3'Nav2
TS28: hippocampus	Present		2_P7 WT 3'Nav2
TS28: main olfactory bulb	Present		2_P7 WT 3'Nav2
TS28: thalamus	Present		2_P7 WT 3'Nav2
TS28: brain	Present	Non-Uniform	2_P7 WT 3'Nav2	Lighter staining was noted throughout many regions of the brain.
TS28: brain white matter	Absent		2_P7 WT 3'Nav2
TS28: caudate-putamen	Absent		2_P7 WT 3'Nav2

Structure	Level	Pattern	Image	Note
TS28: brain	Trace		2_P7 Hyp 3'Nav2	Very little staining was observed.
TS28: cerebellum	Strong	Regionally restricted	2_P7 Hyp 3'Nav2	With the 3' probe, strongly labeled cells were noted at the base of the cerebellum in the Hyp mutant as in wild type. As these were not seen with the 5' probe, these represent the shorter transcript.

Structure	Level	Pattern	Image	Note
TS28: brain	Weak		2_P17 WT 3'Nav2
TS28: cerebral cortex	Weak		2_P17 WT 3'Nav2
TS28: thalamus	Weak		2_P17 WT 3'Nav2

Structure	Level	Pattern	Image	Note
TS28: brain	Trace		2_P17 Hyp 3'Nav2	Very little staining was observed.

Structure	Level	Pattern	Image	Note
TS28: brain	Weak		2_Adult WT 3'Nav2
TS28: cerebral cortex	Weak		2_Adult WT 3'Nav2
TS28: thalamus	Trace		2_Adult WT 3'Nav2

Structure	Level	Pattern	Image	Note
TS28: brain	Trace		2_Adult Hyp 3'Nav2	Very little staining was observed.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	4A''	Expression was detected in the region of the cerebellar primordium where cerebellar granule cell precursor cells were found.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	4B''	Expression was detected in the region of the cerebellar primordium where cerebellar granule cell precursor cells were found.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present	Regionally restricted	5A , 5A'	Expression was detected in large cells in the fiber tracts from vermal sections and in the brainstem. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.
TS28: cerebellum vermis	Present	Regionally restricted	5A , 5A'	Expression was detected in large cells in the fiber tracts from vermal sections and in the brainstem. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present	Regionally restricted	5B' , 5B	Expression was detected in large cells in the fiber tracts from vermal sections and brainstem, as in wild type. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.
TS28: cerebellum vermis	Present	Regionally restricted	5B' , 5B	Expression was detected in large cells in the fiber tracts from vermal sections and brainstem, as in wild type. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	S1E12.5 WT 3'Nav2
TS20: cerebral cortex	Absent		S1E12.5 WT 3'Nav2
TS20: cerebellum primordium	Present	Regionally restricted	S1E12.5 WT 3'Nav2	Expression was detected where the granule cell precursors are found.

Structure	Level	Pattern	Image	Note
TS20: brain	Trace		S1E12.5 Hyp 3'Nav2
TS20: cerebellum primordium	Absent		S1E12.5 Hyp 3'Nav2

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	S1E14.5 WT 3'Nav2
TS22: cerebellum	Present	Regionally restricted	S1E14.5 WT 3'Nav2	Expression was detected where the granule cell precursors are found.
TS22: telencephalon	Present		S1E14.5 WT 3'Nav2

Structure	Level	Pattern	Image	Note
TS22: brain	Trace		S1E14.5 Hyp 3'Nav2
TS22: cerebellum	Absent		S1E14.5 Hyp 3'Nav2
TS22: telencephalon	Absent		S1E14.5 Hyp 3'Nav2