Probe:	5'Nav2
Probe preparation:	Antisense labelled with horseradish peroxidase RNA

Structure	Level	Pattern	Image	Note
TS25: cerebellum granule cell layer	Present		2_E17.5 WT 5'Nav2
TS25: cerebral cortex	Present		2_E17.5 WT 5'Nav2
TS25: hippocampus	Present		2_E17.5 WT 5'Nav2
TS25: main olfactory bulb	Present		2_E17.5 WT 5'Nav2
TS25: thalamus	Present		2_E17.5 WT 5'Nav2
TS25: brain	Present	Non-Uniform	2_E17.5 WT 5'Nav2	Lighter staining was noted throughout many regions of the brain.
TS25: brain white matter	Absent		2_E17.5 WT 5'Nav2
TS25: caudate-putamen	Absent		2_E17.5 WT 5'Nav2

Structure	Level	Pattern	Image	Note
TS25: brain	Trace		2_E17.5 Hyp 5'Nav2	A near absence of staining was observed.

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present		2_P0 WT 5'Nav2	Expression peaked around birth.
TS27: cerebral cortex	Present		2_P0 WT 5'Nav2	Expression peaked around birth.
TS27: hippocampus	Present		2_P0 WT 5'Nav2	Expression peaked around birth.
TS27: main olfactory bulb	Present		2_P0 WT 5'Nav2	Expression peaked around birth.
TS27: thalamus	Present		2_P0 WT 5'Nav2	Expression peaked around birth.
TS27: brain	Present	Non-Uniform	2_P0 WT 5'Nav2	Lighter staining was noted throughout many regions of the brain.
TS27: brain white matter	Absent		2_P0 WT 5'Nav2
TS27: caudate-putamen	Absent		2_P0 WT 5'Nav2

Structure	Level	Pattern	Image	Note
TS27: brain	Trace		2_P0 Hyp 5'Nav2	A near absence of staining was observed.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		2_P7 WT 5'Nav2
TS28: cerebral cortex	Present		2_P7 WT 5'Nav2
TS28: hippocampus	Present		2_P7 WT 5'Nav2
TS28: main olfactory bulb	Present		2_P7 WT 5'Nav2
TS28: thalamus	Present		2_P7 WT 5'Nav2
TS28: brain	Present	Non-Uniform	2_P7 WT 5'Nav2	Lighter staining was noted throughout many regions of the brain.
TS28: brain white matter	Absent		2_P7 WT 5'Nav2
TS28: caudate-putamen	Absent		2_P7 WT 5'Nav2

Structure	Level	Pattern	Image	Note
TS28: brain	Trace		2_P7 Hyp 5'Nav2	A near absence of staining was observed.

Structure	Level	Pattern	Image	Note
TS28: brain	Weak		2_P17 WT 5'Nav2
TS28: cerebral cortex	Weak		2_P17 WT 5'Nav2
TS28: thalamus	Weak		2_P17 WT 5'Nav2

Structure	Level	Pattern	Image	Note
TS28: brain	Trace		2_P17 Hyp 5'Nav2	A near absence of staining was observed.

Structure	Level	Pattern	Image	Note
TS28: brain	Weak		2_Adult WT 5'Nav2
TS28: cerebral cortex	Weak		2_Adult WT 5'Nav2
TS28: thalamus	Trace		2_Adult WT 5'Nav2

Structure	Level	Pattern	Image	Note
TS28: brain	Trace		2_Adult Hyp 5'Nav2	A near absence of staining was observed.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	4A'	Expression was detected in the region of the cerebellar primordium where cerebellar granule cell precursor cells were found.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	4B'	Expression was detected in the region of the cerebellar primordium where cerebellar granule cell precursor cells were found.

Structure	Level	Pattern	Image	Note
TS25: cerebellum external granule cell layer	Present		4C' , 4C

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present	Regionally restricted	4D , 4D'	Expression was most densely expressed in the postmitotic premigratory granule cells, the premigratory layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Regionally restricted	4E , 4E'	Expression was most densely expressed in the postmitotic premigratory granule cells, the premigratory layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		4F , 4F'
TS28: cerebellum molecular layer	Absent		4F' , 4F

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Weak	Diffuse	5C' , 5C
TS28: cerebellum white matter	Weak	Diffuse	5C' , 5C	Diffuse, low-level expression was detected in the fiber tracts.

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Present	Regionally restricted	6C	Expression was detected in cells forming the cornu ammonis. Positive cells were also seen in the intermediate zone.
TS25: dentate gyrus	Present	Regionally restricted	6C	Expression was detected in a small number of cells forming the upper blade of the developing dentate gyrus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus layer	Present	Regionally restricted	6D	Expression was observed throughout the cornu ammonis.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	6E	Expression was distributed throughout the cornu ammonis.
TS28: dentate gyrus	Present	Regionally restricted	6E' , 6E	Expression was detected in the outer shells of the developing blades of the dentate gyrus.
TS28: dentate gyrus hilus	Present	Regionally restricted	6E , 6E'	Expression was detected in a few cells in the region of the dentate gyrus migration stream in the hilus region.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	6F' , 6F	Expression was detected in a larger fraction of each blade at postnatal day 17.

Structure	Level	Pattern	Image	Note
TS28: hippocampus layer	Weak	Regionally restricted	6G	Light expression was observed in cells throughout the cornu ammonis.
TS28: dentate gyrus	Weak		6G' , 6G	Light expression was observed in cells throughout the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Absent		7A , 7A'

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	7B' , 7B	Expression was most apparent where neuroepithelial cells had exited the germinal compartments and were differentiating and migrating toward the nascent cortical plate.
TS22: cerebral cortex marginal layer	Absent		7B' , 7B	No staining was detected in the molecular zone or outermost layer of the cortex.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Non-Uniform	7C' , 7C	At E17.5, staining was seen throughout the developing cortex.
TS25: cortical plate	Strong	Non-Uniform	7C' , 7C	Strongest staining was noted at the upper and lower edges of the cortical plate.
TS25: cerebral cortex subventricular zone	Present		7C' , 7C	Expression was detected in cells exiting the ventricular zone/subventricular zone.
TS25: cerebral cortex ventricular layer	Present		7C' , 7C	Expression was detected in cells exiting the ventricular zone/subventricular zone.

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Present	Widespread	7D' , 7D
TS27: cerebral cortex subventricular zone	Present	Regionally restricted	7D , 7D'	Expression was detected in cells exiting the ventricular zone/subventricular zone.
TS27: cerebral cortex ventricular zone	Present	Regionally restricted	7D , 7D'	Expression was detected in cells exiting the ventricular zone/subventricular zone.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	7E , 7E'
TS28: cortical layer II	Strong		7E , 7E'
TS28: cortical layer III	Strong		7E , 7E'
TS28: cerebral hemisphere white matter	Absent		7E , 7E'

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present		8B , 8A

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present		8C

Structure	Level	Pattern	Image	Note
TS25: thalamus	Present		8E	Expression was most dense in a region of the thalamus adjacent to the hippocampus.

Structure	Level	Pattern	Image	Note
TS27: thalamus	Present		8F	Expression was most dense in a region of the thalamus adjacent to the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Weak		8G	By P7, staining began to dissipate. Expression was most dense in a region of the thalamus adjacent to the hippocampus.

Structure	Level	Pattern	Image	Note
TS25: thalamus	Present		8H

Structure	Level	Pattern	Image	Note
TS27: thalamus	Present		8I

Structure	Level	Pattern	Image	Note
TS28: thalamus	Weak		8J	By P7, staining began to dissipate.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	S1E12.5 WT 5'Nav2
TS20: cerebral cortex	Absent		S1E12.5 WT 5'Nav2
TS20: cerebellum primordium	Present	Regionally restricted	S1E12.5 WT 5'Nav2	Expression was detected where the granule cell precursors are found.

Structure	Level	Pattern	Image	Note
TS20: brain	Trace		S1E12.5 Hyp 5'Nav2
TS20: cerebellum primordium	Absent		S1E12.5 Hyp 5'Nav2

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Widespread	S1E14.5 WT 5'Nav2
TS22: cerebellum	Present	Regionally restricted	S1E14.5 WT 5'Nav2	Expression was detected where the granule cell precursors are found.
TS22: telencephalon	Present		S1E14.5 WT 5'Nav2

Structure	Level	Pattern	Image	Note
TS22: brain	Trace		S1E14.5 Hyp 5'Nav2
TS22: cerebellum	Absent		S1E14.5 Hyp 5'Nav2
TS22: telencephalon	Absent		S1E14.5 Hyp 5'Nav2