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Gene Expression Data
Assay Details
Assay
Reference: J:149502 Hill P, et al., A SHH-independent regulation of Gli3 is a significant determinant of anteroposterior patterning of the limb bud. Dev Biol. 2009 Apr 15;328(2):506-16
Assay type: RNA in situ
MGI Accession ID: MGI:6404100
Gene symbol: Hoxd12
Gene name: homeobox D12
Probe: Hoxd12 probe15
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4G: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 4G Expression restricted to posterior proximal mesenchyme.
Specimen 4H: embryonic day 10.5; Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 4H Authors report weakened posterior restriction of expression. Expression extended anteriorly in distal mesenchyme.
Specimen 4I: embryonic day 10.5; Gli3tm1Urt/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 4I Normal posterior restriction of expression.
Specimen 5D: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: forelimb digit mesenchyme Present Regionally restricted 5D Expression in all digit condensations except digit 1.
TS20: forelimb digit 1 Absent 5D
Specimen 5E: embryonic day 12.5; Gli3Xt-J/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS20: forelimb digit mesenchyme Present Widespread 5E Expression equally strong in all digit condensations.
TS20: forelimb digit 1 Present 5E Expression expanded into anterior-most digit condensation.
Specimen 5F: embryonic day 12.5; Gli3tm1Urt/Gli3Xt-J (more )
Structure Level Pattern Image Note
TS20: forelimb digit mesenchyme Present Regionally restricted 5F Continuous expression only in four posterior digit condensations.
TS20: forelimb digit 1 Absent 5F Anterior region devoid of expression larger than in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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