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Gene Expression Data
Assay Details
Assay
Reference: J:284147 Velecela V, et al., Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4. Development. 2019 Oct 17;146(20):dev178723
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6401370
Gene symbol: Podxl
Gene name: podocalyxin-like
Antibody: anti-Podxl (R&D; AF1556)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4C Control: embryonic day 15.5; Wt1tm1.1Ndha/? (more )
Note: Littermate control; exact genotype not reported. The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS23: visceral pericardium Present 4C Podxl Control Epicardial cells have a flattened and elongated morphology in the controls.
Specimen 4C epiWt1KO: embryonic day 15.5; Wt1tm1.1Ndha/Wt1tm1Nhsn, Tg(GATA5-cre)1Krc/0 (more )
Note: Conditional mutant. The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS23: visceral pericardium Present 4C Podxl epiWT1KO Epicardial cells have a cuboidal shape in the mutants.
Specimen 4F E11.5: embryonic day 11.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS19: visceral pericardium Present 4F Podxl E11.5 Epicardial cells have a cuboidal shape at this stage of development.
Specimen 4F E13.5: embryonic day 13.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS21: visceral pericardium Present 4F Podxl E13.5 Epicardial cells have begun to elongate at this stage of development.
Specimen 4F E15.5: embryonic day 15.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS23: visceral pericardium Present 4F Podxl E15.5 Epicardial cells have a flattened shape at this stage of development.
Specimen 4F E18.5: embryonic day 18.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS26: visceral pericardium Present 4F Podxl E18.5 A squamous epicardium is present at this stage of development.
Specimen 7B left: embryonic day 11.5 (more )
Note: The text of the paper reports the age of this specimen as E12.5. Double labeled: green - Podxl; red - Wt1 (assay MGI:6401394); also stained with Hoechst (blue).
Structure Level Pattern Image Note
TS19: pleura Present 7B left Expression is seen in the lung mesothelium; the cells are cuboidal at this stage of development.
Specimen 7B right: embryonic day 18.5 (more )
Note: Double labeled: green - Podxl; red - Wt1 (assay MGI:6401394); also stained with Hoechst (blue).
Structure Level Pattern Image Note
TS26: pleura Present 7B right Expression is seen in the lung mesothelium; the cells are squamous at this stage of development.
Specimen S5A - E11.5: embryonic day 11.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS19: visceral pericardium Present S5A E11.5 Epicardial cells have a cuboidal shape at this stage of development.
Specimen S5A - E13.5: embryonic day 13.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS21: visceral pericardium Present S5A E13.5 Epicardial cells have begun to elongate at this stage of development.
Specimen S5A - E15.5: embryonic day 15.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS23: visceral pericardium Present S5A E15.5 Epicardial cells have a flattened shape at this stage of development.
Specimen S5A - E18.5: embryonic day 18.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS26: visceral pericardium Present S5A E18.5 A squamous epicardium is present at this stage of development.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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