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Gene Expression Data
Assay Details
Assay
Reference: J:266583 Baumgartner M, et al., Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development. 2018 Aug 28;145(17):dev166322
Assay type: RNA in situ
MGI Accession ID: MGI:6400707
Gene symbol: Rnu11
Gene name: U11 small nuclear RNA
Probe: U11 probe1
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2A Control: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS20: neocortex Present 2A ctrl Expression was detected in the pallium.
Specimen 2A Mutant: embryonic day 11.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS20: neocortex Present 2A mut At E11, sporadic loss of staining was noted in the mutant pallium without a significant increase in TUNEL+ cells.
Specimen 2B Control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS20: neocortex Present 2B ctrl Expression was detected in the pallium.
Specimen 2B Mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS20: neocortex Present 2B mut Onset of cell death was observed at E12, with a significant increase in TUNEL+ cells.
Specimen 2C Control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS21: neocortex Present 2C ctrl Expression was detected in the pallium.
Specimen 2C Mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS21: neocortex Present 2C mut
Specimen 2D Control: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS22: neocortex Present 2D ctrl Expression was detected in the pallium.
Specimen 2D Mutant: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: white - Rnu11; red - TUNEL.
Structure Level Pattern Image Note
TS22: neocortex Present 2D mut
Specimen S2A E12 control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: white - Rnu11; green - Mki67 (assay MGI:6400719); magenta - Rbfox3 (assay MGI:6400724).
Structure Level Pattern Image Note
TS20: neocortex Present S2A E12 ctrl middle , S2A E12 ctrl bottom Expression was detected in the pallium.
Specimen S2A E12 mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: white - Rnu11; green - Mki67 (assay MGI:6400719); magenta - Rbfox3 (assay MGI:6400724).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S2A E12 mut middle , S2A E12 mut bottom Rnu11 FISH in conjunction with immunolabeling showed the presence of Rnu11-null neural progenitor cells (Mki67+) and Rnu11-null neurons (Rbfox3+) in the mutant pallium.
Specimen S2A E13 control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: white - Rnu11; green - Mki67 (assay MGI:6400719); magenta - Rbfox3 (assay MGI:6400724).
Structure Level Pattern Image Note
TS21: neocortex Present S2A E13 ctrl bottom , S2A E13 ctrl middle Expression was detected in the pallium.
Specimen S2A E13 mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: white - Rnu11; green - Mki67 (assay MGI:6400719); magenta - Rbfox3 (assay MGI:6400724).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2A E13 mut middle , S2A E13 mut bottom Rnu11 FISH in conjunction with immunolabeling showed the presence of Rnu11-null neural progenitor cells (Mki67+) and Rnu11-null neurons (Rbfox3+) in the mutant pallium.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory