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Gene Expression Data
Assay Details
Assay
Reference: J:228519 Jia S, et al., Insm1 controls the differentiation of pulmonary neuroendocrine cells by repressing Hes1. Dev Biol. 2015 Dec 1;408(1):90-8
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6391189
Gene symbol: Insm1
Gene name: insulinoma-associated 1
Reporter: lacZ
Antibody: anti-Insm1
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-beta-galactosidase MGI:6391181 may have been used in this assay.
Results
Specimen 1B +/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B +/lacZ (upper) Expression in neuroendocrine cells of distal lung.
Specimen 1B lacZ/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B lacZ/lacZ (upper) Expression in neuroendocrine cells of distal lung.
Specimen 1B +/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B +/lacZ (lower) Expression in neuroendocrine cells of distal lung.
Specimen 1B lacZ/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B lacZ/lacZ (lower)
Specimen 1C +/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C +/lacZ (upper) Expression in proximal and distal lung epithelium.
Specimen 1C lacZ/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C lacZ/lacZ (upper) Expression in proximal and distal lung epithelium.
Specimen 1C +/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C +/lacZ (lower) Expression in proximal and distal lung epithelium.
Specimen 1C lacZ/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C lacZ/lacZ (lower) Expression in proximal and distal lung epithelium.
Specimen 2A +/lacZ: embryonic day 14.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS22: lung epithelium Present 2A +/lacZ (left)
Specimen 2A lacZ/lacZ: embryonic day 14.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS22: lung epithelium Present 2A lacZ/lacZ (left)
Specimen 2B +/lacZ: embryonic day 16.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS24: lung epithelium Present 2B +//lacZ (left)
Specimen 2B lacZ/lacZ: embryonic day 16.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS24: lung epithelium Present 2B lacZ/lacZ (left)
Specimen 2C +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present 2C +/lacZ (left)
Specimen 2C lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present 2C lacZ/lacZ (left)
Specimen 3A +/lacZ: embryonic day 13.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS21: lung epithelium Present 3A +/lacZ
Specimen 3A lacZ/lacZ: embryonic day 13.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS21: lung epithelium Present 3A lacZ/lacZ
Specimen 3B +/lacZ: embryonic day 15.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS23: lung epithelium Present 3B +/lacZ
Specimen 3B lacZ/lacZ: embryonic day 15.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS23: lung epithelium Present Regionally restricted 3B lacZ/lacZ Authors report co-expression with Hes1.
Specimen 3C +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS26: lung epithelium Present 3C +/lacZ
Specimen 3C lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 3C lacZ/lacZ Authors report co-expression with Hes1.
Specimen 4A +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Triple labeled: red - Insm1 reporter; green - CC10 (Scgb1a1 assay MGI:6391194); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4A +//lacZ No co-expression with CC10 (Scgb1a1).
Specimen 4A lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Triple labeled: red - Insm1 reporter; green - CC10 (Scgb1a1 assay MGI:6391194); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4A lacZ/lacZ No co-expression with CC10 (Scgb1a1).
Specimen 4B +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Triple labeled: red - Insm1 reporter; green - SSEA1 (Fut4 assay MGI:6391195); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4B +/lacZ No co-expression with SSEA1 (Fut4).
Specimen 4B lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Triple labeled: red - Insm1 reporter; green - SSEA1 (Fut4 assay MGI:6391195); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4B lacZ/lacZ No co-expression with SSEA1 (Fut4).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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