Gene Expression Data
Assay Details
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Assay
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Reference:
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J:272623
Kinoshita-Kawada M, et al., A crucial role for Arf6 in the response of commissural axons to Slit. Development. 2019 Feb 4;146(3):dev172106
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6390947
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Gene symbol:
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Robo1
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Gene name:
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roundabout guidance receptor 1
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Antibody:
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Robo1 (20219-1-AP)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The authors did not specify which antibody was used, they may have used Robo1 antibody (MGI:6390797) or anti-Robo1 (RP2791)
(MGI:6390805).
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Results
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Specimen
4A wild-type:
embryonic day 12.5
(more  )
Specimen
4A wild-type:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4A Wild-type
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Expression is in the ventral funiculus, post-crossing axon tracts, and is low in pre- and midline-crossing axon segments at brachial-level spinal cords.
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Specimen
4A Arf6-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Arf6tm1Yaka/Arf6tm1Yaka
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Weak |
Regionally restricted |
4A Arf6-/-
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Expression in this mutant was reduced in the ventral funiculus and pre-crossing axonal regions.
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Analysis Tools
